中国药业2018，Vol.27Issue(1)：17-20,4.DOI:10.3969/j.issn.1006-4931.2018.01.005
桔梗总皂苷对PM2.5致肺损伤大鼠表面活性物质相关蛋白A表达的影响
Effect of Platycodon Grandiflorum Saponins on SP-A Expression in Rats with Lung Injury Induced by PM2. 5
摘要
Abstract
Objective To observe the effect of Platycodon grandiflorum saponins(PGS) on the expression of surfatcant - associated protein A ( SP - A ) in rats with lung injury induced by PM2. 5, and to investigate its repair effect on lung injury induced by PM2. 5. Methods Totally 50 Wistar rats (male and female in half, weighing 180 - 220 g) were randomly divided into 5 groups: blank control group, model control group, PGS high, middle and low dose group, 10 rats in each group. The models were established by tracheal instillation of PM2. 5. After administration of 14 d, the middle lobe of left lung tissues and right lung tissues were taken and stained with conventional HE, the pathological changes of lung tissue were observed under microscope. The expression of SP - A in the lung tissues was detected by Western blot(WB), the expression of mRNA in the lung tissues was detected by real - time quantitative PCR (RT - PCR) . BALF and serum of the rats were collected, and the content of SP - A in BALF and serum were determined by ELISA. Results PGS can significantly inhibit inflammation of lung tissue that damaged by PM2. 5, increase the expression of SPA in lung tissue and the content of SPA in BALF, but decrease the content of SPA in serum of rats. Conclusion PGS can repair the type Ⅱalveolar epithelial cells(AEC - Ⅱ) and capillary in lung tissue that damaged by PM2. 5.关键词
桔梗总皂苷/PM2.5/肺泡Ⅱ型上皮细胞/表面活性相关蛋白A/大鼠Key words
Platycodon grandiflorum saponins/PM 2. 5/type Ⅱ alveolar epithelial cell/surfatcant - associated protein A/rat分类
医药卫生引用本文复制引用
姚琳,张俊威,孟庆杰,董坤,王伟明..桔梗总皂苷对PM2.5致肺损伤大鼠表面活性物质相关蛋白A表达的影响[J].中国药业,2018,27(1):17-20,4.基金项目
黑龙江省青年科学基金项目[QC2014C115]. （）
