摘要
Abstract
Objective To improve the method of content determination of phenylbutazone in Phenylbutazone Tablets. Methods The Agilent Eclipse XDB - C18 column(250 mm × 4. 6 mm, 5 μm) was adopted, the mobile phase was sodium acetate buffer (2. 72 g sodium acetate was taken and dissolved with 700 mL of water, the pH was adjusted to 4. 1 with glacial acetic acid, the water was added to 1000 mL and shaken well ) - acetonitrile ( 30 : 70, V / V ) , the flow rate was 1. 0 mL / min, the detection wavelength was 240 nm, the column temperature was 30 ℃ , and the injection volume was 20 μL. Results Phenylbutazone could effective separate from the known impurities. The linear range of phenylbutazone was 0. 01 - 0. 30 g / L ( r = 0. 9999 ) ; RSDs of precision, stability and reproducibility tests were lower than 2. 0％ ( n = 6 ) , the recovery was 99. 00％ - 101. 66％ , RSD = 1. 41％ ( n = 9) . Conclusion The improved method is more sensitive and reproducible, which is suitable for content determination of phenylbutazone in Phenylbutazone Tablets.关键词
高效液相色谱法/保泰松片/保泰松/含量测定/杂质分离/方法改进Key words
HPLC/Phenylbutazone Tablets/phenylbutazone/content determination/impurities separation/method improvement分类
医药卫生
