中国肿瘤生物治疗杂志2017，Vol.24Issue(12)：1370-1374,5.DOI:10.3872/j.issn.1007-385x.2017.12.005
NRP-1单抗联合节律化疗对裸鼠胃癌移植瘤生长的抑制
NRP-1 monoclonal antibody in combination with metronomic chemotherapy (MCT) inhibits the growth of gastric cancer xenografts in nude mice
摘要
Abstract
Objective:To investigate the antitumor effect of NRP-1 monoclonal antibody combined with docetaxel metronomic chemotherapy in nude mice bearing gastric cancer xenografts.Methods:BALB / c mice were inoculated subcutaneously with BGC-823 cells to establish xenograft model;the tumor bearing rats were randomly divided into control group,NRP-1 monoclonal antibody group (NRP-lmAb),rhythm chemotherapy group (MCT) and combined group (NRP-lmAb+MCT).Except the control group,the other three groups were given the corresponding treatment on the 8th day after the model establishment.After 2 weeks of administration,the general condition of nude mice was observed and the body weight and tumor volume were measured the next day.The mice were sacrificed and the mass of tumor was calculated;HE staining was used to observe the morphology of the tumor tissue.The expression of NRP-1 protein,VEGF and MVD were detected by immunohistochemistry.Results:The tumor volume (0.613±0.223 vs 0.866±0.115,1.098±0.343,1.474±0.644 V/cm3;P＜0.05) and weight (0.394±0.128 vs 0.748±0.152,0.867±0.361,1.247±0.494 m/g;P＜0.05) of the combined group were significantly lower than those of the other groups,and the tumor inhibition rate was statistically higher compared with the other treatment groups (P＜0.05).Under microscope,the cancer cells in the control group were well grown and the blood vessels were rich;the cancerous tissues of each treatment group showed different degree of sheet necrosis and the blood vessel composition decreased.Immunohistochemistry results showed that the expression of NRP-1 in the control group was significantly higher than that in each treatment group (P＜0.05);and the expression of NRP-1,VEGF and MVD in combined treatment group was significantly lower than the other groups.Conclusion:NRP-1 monoclonal antibody combined with docetaxel rhythmic chemotherapy may significantly inhibit the growth and angiogenesis of BGC-823gastric cancer by down-regulating the expression of NRP-1.关键词
胃癌/裸鼠移植瘤/抗NRP-1单克隆抗体/节律化疗/血管内皮生长因子Key words
gastric cancer/transplanted tumor in nude mice/NRP-1 mAb/metronomic chemotherapy(MCT)/vascular endothelial growth factor(VEGF)分类
医药卫生引用本文复制引用
丁园,徐芸,陈玉强,颜江华..NRP-1单抗联合节律化疗对裸鼠胃癌移植瘤生长的抑制[J].中国肿瘤生物治疗杂志,2017,24(12):1370-1374,5.基金项目
厦门市科技计划创新项目资助(No.3502220134026,No.3502220144034).Project supported by the Science and Technology Innovation Programme of Xiamen (No.3502220134026,No.3502220144034) （No.3502220134026,No.3502220144034）
