中国肿瘤生物治疗杂志2017，Vol.24Issue(12)：1381-1385,5.DOI:10.3872/j.issn.1007-385x.2017.12.007
MGBA负载脐带血DC诱导特异性CTL对乳腺癌细胞的杀伤作用
MGBA carrying umbilical cord blood-derived dendritic cells induces specific cytotoxic T lymphocytes against breast cancer cells
摘要
Abstract
Objective:To investigate the in vitro cytotoxicity of cytotoxic T lymphocytes (CTLs) induced from umbilical cord blood derived dendritic cells (DCs) that sensitized by mammaglobin A (MGBA) (MGBAp-DCs) against breast cancer.Methods:Umbilical cord blood (UCB) samples were obtained from healthy pregnant women during cesarean delivery and used to isolate UCB mononuclear cells to induce DC.MGBAp-DCs were co-cultured with autologous lymphocytes to induce CTL.The DC phenotypes (CD83,CD86,HLA-DR) were determined by using flow cytometry;and IL-10,IL-12 secretion levels was determined by ELISA;the killing effect of CTL against breast cancer cells was detected by CCK-8.Results:Mature DCs were successfully isolated with typical morphology and functionality characteristics.Specific CTL induced by MGBAp-DCs had a significant cytotoxic effect against breast cancer MDA-MB-415 cells (P＜0.05).Cytotoxic effect was significantly weakened after adding anti-HLA-Ⅰ antibody,but not with adding anti-HLA-Ⅱ antibody.However,the the cytotoxicity of specific CTL on normal breast cell line MCF-10 was not influenced by the presence of anti-HLA-Ⅰ or anti-HLA-Ⅱ antibodies or not.Conclusion:MGBAp-DCs can effectively enhance the cytotocxity of CTL against breast cancer cells in vitro.关键词
脐带血/树突状细胞/乳腺珠蛋白/乳腺癌细胞/细胞毒性T细胞Key words
umbilical cord blood/dendritic cell (DC)/mammaglobin A (MGBA)/breast cancer cell/cytotoxic T lymphocyte (CTL)分类
医药卫生引用本文复制引用
胡园园,刘佩佩,姜又红..MGBA负载脐带血DC诱导特异性CTL对乳腺癌细胞的杀伤作用[J].中国肿瘤生物治疗杂志,2017,24(12):1381-1385,5.基金项目
辽宁省科技厅社会发展攻关计划资助项目(No.2011415052-3). Project supported by the Science and Technology Project of Liaoning Province(No.2011415052-3) （No.2011415052-3）
