中国肿瘤生物治疗杂志2017，Vol.24Issue(12)：1386-1390,5.DOI:10.3872/j.issn.1007-385x.2017.12.008
集落刺激因子1受体介导Bax和Bcl-2表达对人鼻咽癌6-10B细胞凋亡的影响
Colony stimulating factor-1 receptor-mediated Bax/Bcl-2 expression inhibits apoptosis of human nasopharyngeal carcinoma 6-10B cells
摘要
Abstract
Objective:To investigate the inhibitory effect of Colony stimulating factor-1 receptor (CSF-1R) on apoptosis of human nasopharyngeal carcinoma 6-10B cells and its relationship with Bax/Bcl-2 expression.Methods:Lentiviral vector over-expressing CSF-1R LV-CSF1R(16957-1) was constructed and trasfected into 6-10B cells;meanwhile a control group was set;The expression of CSF-1 R,Bcl-2 and Bax was detected by Real-time PCR and Western blotting.CCK-8 assay and flow cytometry (FCM) were used to detect the cell proliferation and apoptosis,respectively.Results:The mRNA expression of CSF-1R increased significantly in 6-10B cells of transfection group compared with negative control (NC) group (7.01±0.23 vs 0.09±0.03,P＜0.01);The expression ofBax mRNA was significantly down-regulated while the expression of Bcl-2 mRNA was significantly up-regulated in 6-10B cells of transfection group (all P＜0.01).The protein expression of CSF-1 in 6-10B cells of transfection group was significantly higher than that of control group,along with the remarkably down-regulated Bax protein expression and slightly up-regulated Bcl-2 protein in transfection group.Compared with NC group,the proliferation vitality of 6-10B cells were extremely increased after transfection (P＜0.01);however,the cell apoptosis was significantly reduced ([10.82±0.75]％ vs [17.11±0.46]％,P＜0.05).Conclusion:Over-expression of CSF-1R could promote the malignant growth and inhibit the apoptosis of nasopharyngeal carcinoma 6-10B cells by regulating the ratio of Bax/Bcl2 expression.关键词
集落刺激因子1受体/Bax/Bcl-2/鼻咽癌/细胞凋亡Key words
colony stimulating factor-1 receptor/Bax/Bcl-2/nasopharyngeal carcinoma/apoptosis分类
医药卫生引用本文复制引用
陈嘉羽,蒿艳蓉,陈甲信,黄俐,敖雯..集落刺激因子1受体介导Bax和Bcl-2表达对人鼻咽癌6-10B细胞凋亡的影响[J].中国肿瘤生物治疗杂志,2017,24(12):1386-1390,5.基金项目
国家自然科学基金资助项目(No.81260348).Project supported by the National Natural Science Foundation of China(No.81260348) （No.81260348）
