中国肿瘤生物治疗杂志2017，Vol.24Issue(12)：1391-1396,6.DOI:10.3872/j.issn.1007-385x.2017.12.009
锌指结构蛋白148可变剪切体在结直肠癌发生发展中的作用
Roles of zinc finger protein 148 alternative splicing isoforms in occurrence and development of colorectal cancer
摘要
Abstract
Objective:To investigate the effect of two alternative splicing isoforms of zinc finger protein 148 (ZNF148) gene on the invasion and metastasis of colon cancer cells and their related mechanisms.Methods:RTPCR was used to determine the expressions of two ZNF148 alternative splicing isoforms in the human colorectal SW480 cells.ZNF148 interference vector and ZNF148 over-expression vector were constructed and transfected into the SW480 cells;them were divided into ZNF148FL-siRNA group,ZNF148FL-Over express group,ZNF148△N-siRNA group,ZNF148△N-Over express group,Control siRNA group,Control Over express group and Normal control group.The mRNA expressions in each group were examined by RT-PCR;the proliferation,invasion and migration in vitro as well as apoptosis of SW480 cells were detected by MTT,Transwell,scratch assay and flow cytometry,respectively.Results:Two splicing isoforms (ZNF148FL of 2 385 bp and ZNF148△N of 2 004 bp) were obtained by RTPCR.The expression level of ZNF148FL was significantly decreased while the expression of ZNF148△N was increased in the ZNF 148FL-siRNA group;the expression of ZNF 148FL was significantly increased while the expression of ZNF148△N was significantly decreased in the ZNF148FL-Over express group (all P＜0.050.The expression of ZNF148△N was significantly decreased while the expression of ZNF148FL was increased in ZNF148△N-siRNA group;the expression of ZNF148△N was significantly increased while the expression of ZNF148FL was decreased in ZNF148△N-over express group (all P＜0.05).The proliferation of the SW480 cells was increased in ZNF148FL-over express group and the ZNF148△N-siRNA group,while the proliferation of the SW480 cells was decreased in the ZNF148FL-siRNA group and the ZNF148△N-Over express group.The transmembrane cell number and migration ability of the SW480 cells in the ZNF148FL-siRNA group and the ZNF148△N-Over express group were significantly decreased,but the apoptotic rate was significantly increased;However,ZNF148FL-Over Express group and ZNF148△N-siRNA group showed the significantly increased transmembrane cell number and migration ability but decreasedapoptosis rate (all P＜0.05).Conclusion:ZNF148FL could increase proliferation,invasion and metastasis of colorectal cancer cells,while ZNF△N showed opposite effect;the two splicing isoforms of ZNF 148 may exert mutual antagonistic effect to each other on the malignant biological activities.关键词
锌指结构蛋白148/可变剪接体/结直肠癌/SW480细胞/侵袭/转移/凋亡Key words
zinc finger protein 148/variable splicing/colorectal cancer/SW480 cell/invasion/metastasis/apoptosis分类
医药卫生引用本文复制引用
何平,高显华,许硕贵,丁杰,况飞..锌指结构蛋白148可变剪切体在结直肠癌发生发展中的作用[J].中国肿瘤生物治疗杂志,2017,24(12):1391-1396,6.基金项目
国家自然科学基金资助项目(No.81572332).Project supported by the National Natural Science Foundation of China (No.81572332) （No.81572332）
