中国肿瘤生物治疗杂志2017，Vol.24Issue(12)：1409-1413,5.DOI:10.3872/j.issn.1007-385x.2017.12.012
胃癌患者血清中miR-133a水平及其与病理特征的相关性
Correlation between serum miR-133a level and pathologic features in gastric cancer patients
摘要
Abstract
Objective:To investigate the correlation between serum miR-133a level and pathological features of gastric cancer,and to explore the diagnostic and prognostic value of miR-133a.Methods:Ninety-four gastric cancer patients treated in the Department of Gastroenterology Surgery of Affiliated Changzhou N0.2 People's Hospital of Nanjing Medical University from March 2010 to April 2011 were included in this study;there were 64 male cases and 30 female cases,aged from 32-82 years old with an mean age of (61.72±8.59) years old;Another 30 healthy subjects were enrolled as control.RT-PCR was used to detect the serum miR-133a expression of two groups.SPSS22.0 and Graphpad5.0 were used for statistical analysis.Results:The levels of serum miR-133a in gastric cancer patients were obviously lower than those in healthy controls (1.95 ± 1.51 vs 4.47 ± 0.56,P=0.000).miR-133 a expression was significantly associated with the degree of differentiation,depth of invasion,lymph node metastasis and TNM stage (P＜0.05),but not obviously associated with age,sex and tumor size (P＞0.05).ROC curve analysis showed high sensitivity and specificity of miR-133a in the prediction of gastric cancer (the AUC value of miR-133a was 0.883,95％CI 0.8257-0.9402) and the prediction of lymph node metastasis (the AUC value of miR-133a was 0.984,95％ CI 0.9633-1.005).The 5-year survival rate of patients with high miR-133a expression was significantly higher than that of low expression group (40.49％ vs 19.37％,P＜0.05).Conclusion:The expression of miRNA-133a was closely associated with gastric cancer development,its clinicopathological features and patient's clinical prognosis.It may be used as a potential diagnostic bio-marker and a prognostic predictor for gastric cancer.关键词
miR-133a/血清/胃癌/TNM分期/预后Key words
miR-133a/serum/gastric cancer/TNM stage/prognosis分类
医药卫生引用本文复制引用
宋军,龚宇,汤黎明,刘寒旸,周艳..胃癌患者血清中miR-133a水平及其与病理特征的相关性[J].中国肿瘤生物治疗杂志,2017,24(12):1409-1413,5.基金项目
常州市科技基础研究项目资助(No.CJ20122014).Project supported by the Changzhou Basic Research Program of Science and Technology(No.CJ20122014) （No.CJ20122014）
