中国肿瘤生物治疗杂志2017，Vol.24Issue(12)：1414-1418,5.DOI:10.3872/j.issn.1007-385x.2017.12.013
非小细胞肺癌患者外周血有核细胞miR-205-5p水平及其临床意义
miR-205-5p expression in peripheral blood karyocytes from non-small cell lung cancer patients and its clinical value
摘要
Abstract
Objective:To investigate whether miR-205-5p expression in peripheral blood karyocytes from nonsmall cell lung cancer (NSCLC) patients could be used as a reasonable molecule biomarker.Methods:Peripheral blood from 60 patients,who were primarily diagnosed with NSCLC and treated at the Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital) from June,2011 to June,2012,was collected;among them,there were 30 cases with distant metastasis while the other 30 without.In addition,30 healthy subjects were enrolled as controls.The miR-205-5p expressions in collected peripheral blood karyocytes,human bronchial epithelial BEAS-2B cells,Gejiu squamous cell lung carcinoma YTMLC-90 cells,and Xuanwei lung adenocarcinoma XWLC-05 cells were detected by qPCR.We also analyzed the correlation between miR-205-5p expression in peripheral blood karyocytes and NSCLC serum markers levels,TNM stage,lymph node metastasis,as well as distant metastasis etc.Results:The expressions of miR-205-5p in YTMLC-90 (17.507 2±1.906 3) and XMLC-05 cells (5.725 2±1.0120) were significantly higher than that in BEAS-2B cells (0.820 8±0.255 3) (P＜0.05).The miR-205-5p expression level in peripheral blood karyocytes from healthy controls was significantly higher than that from NSCLC patients (P＜0.01).The positive rate of miR-205-5p in peripheral blood karyocytes from NSCLC patients was not correlated with patients' sex,age,lymph node metastasis,pathologic subtypes and tumor serum markers (CEA,CA125,CA242) (all P＞0.05),but significantly correlated with distant metastasis,TNM stage and tumor serum marker CA153 (P＜0.01).Conclusion:Abnormal up-regulation of miR-205-5p may play an important role in the progression of NSCLC and can be used as a reasonable biomarker for the metastasis in NSCLC patients.关键词
miR-205-5p/外周血有核细胞/非小细胞肺癌/转移/液体活检Key words
miR-205-5p/peripheral blood mononuclear cells/non-small cell lung cancer/metastasis/liquid biopsy分类
医药卫生引用本文复制引用
陈艳,向敏,王熙才,周永春,金从国,刘馨,姚乾..非小细胞肺癌患者外周血有核细胞miR-205-5p水平及其临床意义[J].中国肿瘤生物治疗杂志,2017,24(12):1414-1418,5.基金项目
国家自然科学基金资助项目(No.81560380,81460358,81060177) （No.81560380,81460358,81060177）
云南省卫生科技项目资助(No.2014NS023,2014NS024) （No.2014NS023,2014NS024）
詹启敏院士工作站基金资助(No.2015IC002).Project supported by the National Natural Science Foundation of China (No.81560380,No.81460358,No.81060177),the Yunnan Provincial Technology Project of Health (No.2014NS023,No.2014NS024),and the Zhan Qimin Academician Workstation of Yunnan Province(No.2015IC002) （No.2015IC002）
