北京中医药大学学报2017，Vol.40Issue(11)：917-922,6.DOI:10.3969/j.issn.1006-2157.2017.11.008
五子衍宗丸对幼年大鼠睾丸支持细胞增殖的影响及机制
Influence and mechanism of Wuzi Yanzong Wan on proliferation of Sertoli cells in infant rats
摘要
Abstract
ObjectiveTo discuss the effect and mechanism of Wuzi Yanzong Wan (Five Seeds for Multiplying Pills) in the proliferation of Sertoli cells in infant rats.Methods The Sertoli cells were separated from male SD rats (20 d old) and cultured,and then randomly divided into normal group,lowdose Wuzi Yanzong Wan group (5 g/L,low-dose group),mid-dose Wuzi Yanzong Wan group (10 g/L,mid-dose group) and high-dose Wuzi Yanzong Wan group (15 g/L,high-dose group).The changes of optical density (OD) of Sertoli cells were detected by using methyl thiazolyl tetrazolium test (MTT') in all groups.The expression of Ki67 positive granules in Sertoli cells were detected by using immunohistochemistry technique in all groups.The expressions of Akt and p-Akt in Sertoli cells were detected by using Western blot method.Results Compared with normal group,the proliferation of Sertoli cells treated with Wuzi Yanzong Wan was promoted significantly (P ＜ 0.05 or P ＜ 0.01).The expression of Ki67 positive granules increased significantly (P ＜ 0.05 or P ＜ 0.01),and relative expression of p-Akt increased significantly (P ＜ 0.05 or P ＜ 0.01).Conclusion Wuzi Yanzong Wan can possibly promote the proliferation of Sertoli cells in infant rats through activating Akt signal transduction pathway and up-regulating p-Akt expression.关键词
五子衍宗丸/支持细胞/体外培养/增殖/Akt信号传导通路/大鼠Key words
Wuzi Yanzong Wan (Five Seeds for Multiplying Pills)/Sertoli cells/culture in vitro/proliferation/Akt signal transduction pathway/rats分类
医药卫生引用本文复制引用
胡素芹,李悦,曹龑,简郭血骄,郭健..五子衍宗丸对幼年大鼠睾丸支持细胞增殖的影响及机制[J].北京中医药大学学报,2017,40(11):917-922,6.基金项目
国家自然科学基金资助项目(No.81273610) National Natural Science Foundation of China (No.81273610) （No.81273610）
