北京中医药大学学报2017，Vol.40Issue(11)：923-927,5.DOI:10.3969/j.issn.1006-2157.2017.11.009
益气活血通络方对糖尿病周围神经病变小鼠JNK信号通路蛋白及炎性因子的影响
Influence of Yiqi Huoxue Tongluo Fang on JNK signal pathway and inflammatory factors in mice with diabetic peripheral neuropathy
摘要
Abstract
ObjectiveTo study the influence of Yiqi Huoxue Tongluo Fang (Qi-Replenishing Blood-Activating Collateral-freeing Decoction) on signal pathway of c-jun N-terminal kinase (JNK) and inflammatory factors in mice with diabetic peripheral neuropathy (db/db mice),and discuss its mechanism in protecting peripheral nerve.Methods The db/db mice (n =40) were randomly divided into 5 groups:model group,lipoic acid group,high-dose Yiqi Huoxue Tongluo Fang group (6.4 g/kg,high-dose group),mid-dose Yiqi Huoxue Tongluo Fang group (3.2 g/kg,mid-dose group) and low-dose Yiqi Huoxue Tongluo Fang group (1.6 g/kg,low-dose group),and other db/m mice (n =8) were chosen into normal group.All groups were intragastrically given corresponding drugs once a day for 8 weeks.After the last administration of drugs,the plantar thermal response time and motor nerve conduction velocity (MNCV) were detected.The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by using enzyme-linked immunosorbent assay (ELISA).The protein expressions of JNK and phosphorylase JNK (p-JNK) in dorsal root ganglion (DRG) were detected by using Western blot method.Results Compared with normal group,MNCV was slower,plantar thermal response time was longer,levels of IL-1β and TNF-α increased,and protein expressions of JNK and pJNK are stronger in model group (P ＜ 0.01 or P ＜ 0.05).Compared with model group,Yiqi Huoxue Tongluo Fang promoted MNCV,shortened plantar thermal response time,decreased levels of IL-1 β and TNF-α and down-regulated protein expressions of JNK and p-JNK to some extent (P ＜ 0.01 or P ＜0.05).ConclusionYiqi Huoxue Tongluo Fang can inhibit the abnormal activation of JNK signal pathway and relieve inflammatory reaction to protect peripheral nerve in db/db mice.关键词
益气活血通络方/db/db小鼠/糖尿病周围神经病变/JNK信号转导通路/炎性因子Key words
Yiqi Huoxue Tongluo Fang (Qi-Replenishing Blood-Activating Collateral-freeing Decoction)/db/db mice/diabetic peripheral neuropathy/JNK signal pathway/inflammatory factors分类
医药卫生引用本文复制引用
姚永传,申国明,江爱娟,王靓,叶树,王柳..益气活血通络方对糖尿病周围神经病变小鼠JNK信号通路蛋白及炎性因子的影响[J].北京中医药大学学报,2017,40(11):923-927,5.基金项目
国家自然科学基金项目(No.81273646),安徽省自然科学基金项目(No.1608085MH232) National Natural Science Foundation(No.81273646),Natural Science Foundation of Anhui Province(No.1608085MH232) （No.81273646）
