草业学报2018，Vol.27Issue(1)：152-160,9.DOI:10.11686/cyxb2017107
甘农三号紫花苜蓿种带细菌的生物功能分析及鉴定
Identification and biological attributes of seed-borne bacteria isolated from lucerne cv.Gannong No.3
摘要
Abstract
Eight bacterial strains (ZSR9-16) were isolated from surface-sterilized seeds of Gannong No.3 lucerne using the dilution plate method.Biological functions including indole acetic acid (IAA) production,nitrogen fixation,phosphate solubilization and ability to decompose cellulose were determined for all strains.The results showed that six strains (ZSR9-12 and ZSR14-15) had the ability to produce IAA,solubilize phosphorus,fix nitrogen and decompose cellulose;ZSR9 was better than other strains.Strain ZSR13 had weak IAA production.ZSR16 had good IAA production,phosphorus solubilization,cellulose decomposition and nitrogen fixation.The morphology,phenotype and 16S rDNA sequence of all 8 strains were analyzed and revealed that they belonged to three different genera.Six Bacillus strains included B.subtilis ZSR9,B.safensis ZSR10,B.mojavensis ZSR11,B.licheniformis ZSR12,B.pumilus ZSR14 and B.atrophaeus ZSR15.ZSR13 and ZSR16 belonged to Enterobacter sp.and Terribacillus sp.,respectively.These strains will be potentially very useful for improving production of lucerne.关键词
甘农三号/种带细菌/芽孢杆菌/土地芽孢杆菌/肠杆菌Key words
lucerne cultivar Gannong No.3/seed-borne bacteria/Bacillus/Terribacillus/Enterobacter引用本文复制引用
张振粉,师尚礼..甘农三号紫花苜蓿种带细菌的生物功能分析及鉴定[J].草业学报,2018,27(1):152-160,9.基金项目
国家自然科学基金(31460639),国家牧草产业技术体系项目(CARS-35)和甘肃省自然科学基金(145RJZA130)资助. （31460639）
