草业学报2018，Vol.27Issue(1)：161-168,8.DOI:10.11686/cyxb2017095
藿香内生细菌HX-2的鉴定、耐性及对宿主植物的促生作用
Identification, tolerance to abiotic stress and host plant effects of endophytic bacteria HX-2 from Agastache rugosa
摘要
Abstract
To identify microbial resources and provide a theoretical basis for high-yielding Agastache rugosa crops,an endophytic bacteria,HX-2,was isolated from healthy leaves of A.rugosa and identified through morphological observation and 16S rDNA sequence analysis.Indole acetic acid production,phosphate solubilization,salt and copper tolerance,and the effects on seed germination and seedling growth of A.rugosa were assessed in vitro and in vivo.The isolated bacterium was identified as Bacillus megaterium which is able to produce indole acetic acid and dissolve inorganic phosphorus.It was tolerant of pH's ranging from 5.0 to 9.0,0.5％ to 8％ NaCl and 0 to 6 mmol · L-1 Cu2+.After inoculation with HX-2,seed germination energy,seed germination percentage,seedling height,root length,leaf area,seedling fresh and dry weights,chlorophyll content,root vigor and stem diameter ofA.rugosa improved by 22.6％,18.0％,17.1％,18.2％,14.7％,22.8％,25.0％,10.0％,19.2％ and 10.4％,respectively (P＜0.05).These results suggest that HX-2 is a growth promoting bacteria which can be developed as a microbial fertilizer for agricultural production.关键词
内生细菌/抗性/促生效应/巨大芽孢杆菌/藿香Key words
endophytic bacteria/resistance/growth-promoting effect/Bacillus megaterium/Agastache rugosa引用本文复制引用
江绪文,李贺勤,谭勇..藿香内生细菌HX-2的鉴定、耐性及对宿主植物的促生作用[J].草业学报,2018,27(1):161-168,8.基金项目
青岛农业大学高层次人才引进项目(1113346),青岛农业大学应用型人才培养特色名校建设工程教学研究项目(XJG2013147),公益性行业(农业)科研专项(201303002)和青岛市自主创新计划(15-9-1-86-jch)资助. （1113346）
