草业学报2018，Vol.27Issue(1)：169-176,8.DOI:10.11686/cyxb2017275
喀斯特山区草地施肥对放牧乌蒙半细毛羊抗氧化系统功能的影响
Effect of nitrogenous fertilizer on the antioxidant systems of grassland species in the Karst mountains
摘要
Abstract
To identify the effect of different nitrogen fertilizer on grassland,fertilization and grazing treatments were applied to perennial grassland grazed by Wumeng semi-fine wool sheep in Weining County.The results showed that copper content in grasses was relatively low,but was higher than the minimum requirement for sheep.Application of nitrogen significantly increase nitrogen content (P＜0.01) in herbage but there were no differences among different forms of nitrogen.Ammonium sulfate significantly increased the sulfur and zinc content (P＜0.01) of herbage,but significantly decreased the selenium content (P＜0.01).At the end of grazing experiment,copper,iron and selenium concentration in the blood of sheep grazing grassland treated with ammonium sulfate was significantly lower (P＜0.01) than in sheep grazing ammonium nitrate treated grassland;blood zinc and sulfur were also significantly higher (P＜0.01).Sheep grazing ammonium sulfate treated pasture had significantly lower (P＜0.01) blood hemoglobin and hematocrit capacity than sheep grazing pasture treated with ammonium nitrate and the control (no fertilizer);antioxidant enzymes including serum ceruloplasmin,serum superoxide dismutase and serum glutathione peroxidase were significantly lower (P＜0.01) than in sheep grazing the pasture treated with ammonium nitrate and the control.Malondialdehyde (MDA) content was also higher (P＜0.01).There were no differences in other blood mineral element concentrations,blood indexes and serum biochemical values.It was concluded that fertilizer selection should be based on soil mineral content;grassland with low copper content is not suitable for ammonium sulfate fertilizer.Ammonium sulfate fertilizer significantly affected the antioxidant system function.关键词
氮肥/喀斯特山区/草地/乌蒙半细毛羊/抗氧化系统功能Key words
nitrogenous fertilizer/karst mountain areas/grassland/Wumeng semi-fine sheep/function of antioxidant system引用本文复制引用
廖建军,申小云,霍宾,熊康宁..喀斯特山区草地施肥对放牧乌蒙半细毛羊抗氧化系统功能的影响[J].草业学报,2018,27(1):169-176,8.基金项目
贵州省研究生教育创新计划项目(黔教研合GZS字[2016]04号),贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2010]101号)和贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2011]17号)资助. （黔教研合GZS字[2016]04号）
