动物医学进展2018，Vol.39Issue(1)：30-33,4.
禽呼肠病毒在鸡胚成纤维细胞系中的增殖规律
Proliferation of Avian Reovirus in Chicken Embryo Fibroblast Cells
摘要
Abstract
To study the proliferation of avian reovirus(ARV)on chicken embryo fibroblasts(DF1),DF1 cells was infected with ARV S1133 strain and serialized continuouly for three passages.The cytopathic effect(CPE)was observed after virus infection,and virus was identified by RT-PCR and the proliferation of the virus was studied by Reed-Meunch method.The results showed that ARV S1133 strain was palpable to DF1 cells and had typical CPE.TCID50was measured at seven time points according to the Reed-Meunch method and plotted as a growth curve,the results revealed that ARV began to proliferate at hour 12,and reached a rapid proliferation during hour 12 to 48 post-infection.The virus titer reached the peak at hour 48,TCID50was 10-8.9/0.1 mL.This study provided a good platform for further study on the pathogenesis of ARV and a theoretical basis for the development of cell-inactivated vaccine of avian reovirus at DF1 cells.关键词
禽呼肠病毒/鸡胚成纤维细胞/病毒效价/一步生长曲线Key words
Avian reovirus/DF1 cell/virus titer/growth curve分类
农业科技引用本文复制引用
沈文康,罗思思,范晴,谢志勤,邓显文,谢芝勋,王盛,黄莉,谢丽基,黄娇玲,曾婷婷,张艳芳,张民秀..禽呼肠病毒在鸡胚成纤维细胞系中的增殖规律[J].动物医学进展,2018,39(1):30-33,4.基金项目
广西自然科学基金项目(2017GXNSFBA198140) （2017GXNSFBA198140）
国家自然科学基金项目(31660715) （31660715）
广西水产畜牧兽医局科技项目(桂渔牧科201452003) （桂渔牧科201452003）
广西兽医生物技术室开放基金项目(14-045-31-A-2) （14-045-31-A-2）
国家"万人计划"领军人才专项项目(2016-37) （2016-37）
中国东盟及一带一路南亚国家动物疫病防控技术研发联合实验室建设项目(桂科AB16380003) （桂科AB16380003）
