动物医学进展2018，Vol.39Issue(1)：34-40,7.
伊犁地区牛源大肠埃希菌血清分型和ERIC-PCR分型研究
Study on Serotyping and ERIC-PCR Genotyping of Bovine Escherichia coli in Yili Area
摘要
Abstract
To investigate the phenotype and genotype of Escherichia coli isolated from beef cattle farms and its designated slaughter and processing field,and to provide scientific basis for the pollution condition of E. coli in the industrial chain,the bovine E.coli was detected on the O antigen with polyvalent serum,the 4 and 5 polyvalent serum strains were selected and detected by PCR(O7,O39,O5,O114,O116,O6,O9,O30, O55)and ERIC-PCR genotyping.The results showed that 4,5,6,9 of polyvalent serotypes of the tested samples were dominant,all more than 5%.The PCR detection results were serotypes of the O5(2 strains), O114(3 strains),O116(4 strains),O6(6 strains),O9(2 strains).ERIC-PCR had 13 genotypes in 4 and 5 polyvalent serotypes,and can be divided into 6 clusters.The similarity was between 59%-100%.The same polyvalent serotype was in a cluster,the D cluster has the 12 strains and the similarity was over 70%.But the different strains in same serotype was not in same cluster genotypes.关键词
牛源大肠埃希菌/O抗原多价血清/聚合酶链反应/ERIC-PCR基因分型Key words
bovine E.coli/O antigen polyvalent serum/PCR/ERIC-PCR genotyping分类
农业科技引用本文复制引用
刘英玉,张妍,刘俊飞,吾买尔江·牙合甫,张晓红,姚刚..伊犁地区牛源大肠埃希菌血清分型和ERIC-PCR分型研究[J].动物医学进展,2018,39(1):34-40,7.基金项目
新疆维吾尔自治区自然科学基金项目(2014211B020) （2014211B020）
新疆维吾尔自治区博士后普通资助项目 （）
