南方医科大学学报2018，Vol.38Issue(1)：1-7,7.
色素上皮衍生因子通过调控上皮间质转化抑制乳腺癌细胞侵袭和转移
Pigment epithelium-derived factor inhibits metastasis and invasion of breast cancer via regulation of epithelial-mesenchymal transition
摘要
Abstract
Objective To investigate whether pigment epithelium-derived factor (PEDF) inhibits invasion and metastasis of breast cancer through regulation of epithelial-mesenchymal transition. Methods The expressions of PEDF, vimentin, and E-cadherin were detected in 119 breast cancer tissues using immunohistochemistry. SK-BR-3 breast cancer cell models of PEDF knockdown and PEDF overexpression were established by transfecting the cells with a PEDF-siRNA vector and a lentivirus-PEDF vector, respectively. Western blotting was used to detect the changes in the expressions of PEDF, vimentin, and E-cadherin in the cells, and the cell invasion and migration ability was assessed using scratch wound healing assay and transwell migration assay. Results PEDF positivity rate was significantly lowered in breast cancer tissues compared with the adjacent tissues. PEDF was positively correlated with the tumor size and the expression level of E-cadherin (r=0.473, P<0.001), but was negatively correlated with vimentin expression (r=-0.412, P<0.001). Transwell invasion experiment showed that PEDF interference enhanced the cell invasion and metastasis, while PEDF overexpression inhibited the invasion and migration of SK-BR-3 cells. Western blotting showed that PEDF knockdown significantly decreased the expression of E-cadherin (P<0.05) and increased vimentin expression in the cells (P<0.05). Conclusion PEDF is closely related with the metastasis of breast cancer cells through regulation of epithelial-mesenchymal transition.关键词
乳腺癌/色素上皮衍生因子/上皮-间质转化/浸润转移Key words
breast cancer/pigment epithelium-derived factor/epithelial-mesenchymal transition/invasion/metastasis引用本文复制引用
周丹,许鹏程,张敏,于洋,吴爱国..色素上皮衍生因子通过调控上皮间质转化抑制乳腺癌细胞侵袭和转移[J].南方医科大学学报,2018,38(1):1-7,7.基金项目
国家自然科学基金(81402613) （81402613）
广东省医学科研基金(B2014395) （B2014395）
佛山市科技局医学科研立项课题(2015AB00307197) Supported by National Natural Science Foundation of China (81402613). （2015AB00307197）
