南方医科大学学报2018，Vol.38Issue(1)：8-13,6.
敲低SORL1表达构建模拟阿尔茨海默病的细胞模型
Establishment of a new cell model mimicking Alzheimer's disease by knocking down SORL1 expression
摘要
Abstract
Objective To establish a cell model mimicking Alzheimer's disease (AD) by knocking down SORL1 gene and compare the viability, apoptosis, and expressions of tumor necrosis factor-α( TNF-α) and interleukin-1β(IL-1β) in this model with a traditional Alzheimer's disease cell model. Methods A traditional cell model of AD was established by inducing N2a cells with Aβ25-35, and the optimal Aβ25-35 concentration was determined by assessing the cell viability changes. Another cell model of AD was established by transfecting N2a cells with SORL1-shRNA lentiviral vector, and SORL1 expression in the transfected cells were detected using Western blotting and qRT-PCR. With wild-type N2a cells without any treatment and cells transfected with a scramble shRNA as the control groups, the two cell models were examined for cell viability with MTT assay, cell apoptosis with flow cytometry, and TNF-αand IL-1βlevels in the culture supernatant with ELISA. Results The two cell models of AD showed obviously decreased viability and increased cell apoptosis compared with the untreated control cells or cells transfected with a scramble shRNA (P<0.05); no significant difference was found in the cell viability and apoptosis rate between the two AD cell models or between the two control groups (P>0.05). Significantly increased expressions of TNF-αand IL-1βwere observed in both of the two cell models compared with their respective control groups (P<0.05) without significant differences between the two cell models or between the two control groups (P>0.05). Conclusion A new AD cell model similar to Aβ25-35-induced AD model can be established by SORL1 knockdown in N2a cells.关键词
分拣蛋白相关受体1/阿尔茨海默病/N2a细胞/转染Key words
SORL1/Alzheimer's disease/N2a cell/transfection引用本文复制引用
罗静,赵艳,谢婧雯,刘鑫,林芳波,侯德仁..敲低SORL1表达构建模拟阿尔茨海默病的细胞模型[J].南方医科大学学报,2018,38(1):8-13,6.基金项目
湖南省科技厅重点研发计划(2016JC2052) （2016JC2052）
中南大学湘雅三医院新湘雅人才工程"至善领跑计划"(20150305) （20150305）
中南大学研究生自主探索创新项目(2017zzts888) （2017zzts888）
