南方医科大学学报2018，Vol.38Issue(1)：62-68,7.
关节腔注射抗坏血酸/氯化铁可延缓骨性关节炎大鼠软骨退变
Intra-articular injection of ascorbic acid/ferric chloride relieves cartilage degradation in rats with osteoarthritis
摘要
Abstract
Objective To assess the effect of ascorbic acid/ferric chloride (AA/FeCl3) in attenuating cartilage damage in rats with osteoarthritis. Methods Thirty adult male Wistar rats with surgically induced osteoarthritis were randomized into 2 groups for treatment with intra-articular injection of saline (control group) or AA/FeCl3 mixture (AA group) once a week starting from the third week after the operation. At 6, 9, and 12 weeks after the operation, 5 rats from each group were sacrificed for observing subchondral bone changes on X-ray films and evaluation of cartilage degeneration in the right knee joints using safranin-O/Fast green staining and a modified OARSI scoring system. The degradation of the cartilage matrix was observed by immunohistochemical staining for type II collagen. Results X-ray examination in saline control group revealed the presence of osteophytes and narrowing of the joint space at 9 weeks, and the joint line disappeared at 12 weeks after the surgery; only slight irregularity of the articular surface was observed in the AA group at 9 and 12 weeks. OARSI scores were significantly lower in AA group than in the control group at 9 weeks (18.67 ± 0.67 vs 12.17 ± 2.75;P<0.05) and 12 weeks (20.11 ± 1.84 vs 13.77 ± 0.40;P<0.05) but not at 6 weeks after the surgery. The content of type 2 collagen in AA group was significantly higher than that in the control group at 6 weeks (0.36 ± 0.039 vs 0.49 ± 0.029;P<0.05) and 9 weeks after the surgery (0.25 ± 0.041 vs 0.38 ± 0.040;P<0.05). Conclusions Early intra-articular injection of AA/FeCl3 can effectively delay the progression of post-traumatic osteoarthritis in rats.关键词
骨性关节炎/抗坏血酸/氯化铁/关节腔注射/芬顿反应/潜伏TGFβ1Key words
osteoarthritis/ascorbic acid/ferric chloride/intra-articular injection/fenton reaction/latent TGFβ1引用本文复制引用
廖哲霆,邢祯全,陈宇璠,邓仲豪,吴德胜,赵亮..关节腔注射抗坏血酸/氯化铁可延缓骨性关节炎大鼠软骨退变[J].南方医科大学学报,2018,38(1):62-68,7.基金项目
国家自然科学基金(31328008) （31328008）
广东省自然科学基金(2014A030313275) （2014A030313275）
广东省自然科学基金(s2013010014253) Supported by National Natural Science Foundation of China (31328008). （s2013010014253）
