南方医科大学学报2018，Vol.38Issue(1)：75-80,6.
人RhoA发生了SUMO化修饰
Human RhoA is modified by SUMO2/3
摘要
Abstract
Objective To investigate whether human RhoA is modified by SUMO. Methods Overlap extension PCR and double digestion technique were used to construct the eukaryotic expression vector pcDNA3-3flag-RhoA, which was identified by sequencing. The plasmid was transfected into HEK293T cells and its expression was detected by Western blotting. Immunofluorescence assay was used to detect whether RhoA is co-localized with SUMO. Co-Immunoprecipitation was used to detect whether RhoA is modified by SUMO. Results The recombinant plasmid pcDNA3-3flag-RhoA was successfully constructed and verified. Western blotting showed that the recombinant plasmid pcDNA3-3flag-RhoA expressed abundant fusion protein in HEK293T cells. Immunofluorescence showed that RhoA was co-localized with SUMO2/3 but not with SUMO1. Co-immunoprecipitation verified that RhoA was modified by SUMO2/3 but not SUMO1. Conclusion Human RhoA is modified by SUMO2/3 and probably participates in the regulation of axon regrowth after nervous system injury.关键词
RhoA/SUMO化修饰Key words
RhoA/SUMOylation引用本文复制引用
万滢聪,李春燕,佘佳瑶,王靖雅,陈明..人RhoA发生了SUMO化修饰[J].南方医科大学学报,2018,38(1):75-80,6.基金项目
国家自然科学基金(31371146,81070983) Supported by National Natural Science Foundation of China (31371146, 81070983). （31371146,81070983）
