南方医科大学学报2018，Vol.38Issue(2)：148-154,7.DOI:10.3969/j.issn.1673-4254.2018.02.05
丝胶蛋白通过自噬通路调控人胃癌MKN45细胞的增殖
Sericin regulates proliferation of human gastric cancer MKN45 cells through autophagic pathway
摘要
Abstract
Objective To investigate the effect of sericin on the proliferation of human gastric cancer MKN45 cells and explore the underlying molecular mechanism. Methods MKN45 cells were transfected by LC3 double fluorescent autophagic virus, and the positive cells screened by puromycin were divided into blank group, sericin group and sericin+3-MA group. After incubation with sericin for 48 h, the cells were examined for proliferation, apoptosis and cell cycle using CCK-8 assay and flow cytometry. Cell autophagy was detected by transmission electron microscopy (TEM) and fluorescent inverted microscope, and the autophagy-related markers including LC3, p62 and Beclin proteins were detected with Western blotting. Nude mice bearing gastric cancer xenograft were treated with normal saline or sericin injections (n=5) and the changes in the tumor volume and weight were measured. Results Compared with the blank group, MKN45 cells with sericin treatment showed significantly inhibited proliferation both in vitro and in nude mice. Autophagosomes were observed in sericin-treated cells under TEM and fluorescent inverted microscope. Sericin treatment of the cells significantly increased the cell apoptosis (P<0.01), caused obvious cell cycle arrest in G2/M phase (P<0.01), up-regulated the expressions of both LC3-2 and Beclin, and down-regulated the expression of p62. The autophagy inhibitor 3-MA obviously antagonized the effects of sericin on cell apoptosis, cell cycle and autophagic protein expressions. Conclusion Sericin can inhibit the proliferation of human gastric cancer MKN45 cells by regulating cell autophagy to serve as potential anti-tumor agent.关键词
丝胶蛋白/自噬/细胞增殖/胃癌Key words
sericin/autophagy/cell proliferation/gastric cancer引用本文复制引用
郭伟洪,陈昭宇,陈豪,林填,赵明利,刘浩,余江,胡彦锋,李国新..丝胶蛋白通过自噬通路调控人胃癌MKN45细胞的增殖[J].南方医科大学学报,2018,38(2):148-154,7.基金项目
广东省大学生攀登计划(pdjh2017a0096) （pdjh2017a0096）
