南方医科大学学报2018，Vol.38Issue(2)：198-204,7.DOI:10.3969/j.issn.1673-4254.2018.02.13
SMU.2055基因对变异链球菌UA159耐酸能力的影响
Role of SMU.2055 gene in regulating acid resistance of Streptococcus mutans UA159
摘要
Abstract
Objective To evaluate the effect of SMU.2055 gene on acid resistance of Streptococcus mutans. Methods A SMU.2055-dificient mutant strain of S. mutans was constructed using homologous recombination technique. The growth of the wild-type and mutant strains was monitored in both normal and acidic conditions. The lethal pH level, glycolysis, proton permeability, cell permeability and biofilm formation of the two strains were compared. Results PCR and sequence analyses verified the successful construction of the SMU.2055-dificient mutant strain. The growth and biofilm formation capacity of the mutant strain were obviously lowered in both normal and acidic conditions. The mutant strain also showed increased lethal pH level, proton permeability, and cell permeability with impaired H+-ATPase activity in acidic conditions, but its minimum glycolytic pH remained unaffected. Conclusion The SMU.2055-deficient S. mutans mutant exhibits a lowered acid resistance, which affects the growth, lethal pH, proton permeability, H +-ATPase activity, cell permeability and biofilm formation but not the minimum glycolytic pH of the mutant strain.关键词
变异链球菌/乙酰基转移酶/SMU.2055基因/耐酸性Key words
Streptococcus mutans/acetylase/SMU.2055 gene/acid resistance引用本文复制引用
李转玲,许晓虎,陈璇,吴昕彧,赵望泓..SMU.2055基因对变异链球菌UA159耐酸能力的影响[J].南方医科大学学报,2018,38(2):198-204,7.基金项目
深圳市科技计划项目(JCYJ20160428142231354) （JCYJ20160428142231354）
