南方医科大学学报2018，Vol.38Issue(2)：205-210,6.DOI:10.3969/j.issn.1673-4254.2018.02.14
实验树鼩心肌缺血再灌注离体模型的构建
Establishment of an ex vivo myocardial ischemia-reperfusion model in tree shrews
摘要
Abstract
Objective To establish an ex vivo model of myocardial ischemia reperfusion in tree shrews. Methods The Langendorff ex vivo heart perfusion system was used to establish the myocardial ischemia reperfusion model in tree shrews with different irrigation and reperfusion time settings. Alanine aminotransferase (ALT), aspartate transaminase (AST) and lactic dehydrogenase (LDH) levels were measured by enzyme-labeled immunosorbent assay, creatine kinase MB (CK-MB) was detected using immunosuppression method, and malondialdehyde was measured with thiobarbital staining method; the infarct size was measured using 2, 3, 5-triphenyltrazoliumchloride (TTC) method. Results Ischemia for 30 min and reperfusion for 30 and 60 min caused more significant increase in CK-MB and LDH levels in the perfusion fluid and also in the levels of ALT, CK-MB and AST in the myocardial tissue compared with other experimental settings (P<0.05), but these parameters were comparable between the former two settings (P>0.05). The mean heart rate in 30-min ischemia with 60-min reperfusion group was obviously lower than that in continuous reperfusion group, 15-min ischemia with 30-min reperfusion group and 30-min ischemia with 30-min reperfusion group (P<0.05), and the heart rate was similar between the latter 3 groups (P>0.05). ECG analysis showed that the mean heart rate in 30-min ischemia with 30-min reperfusion group was closer to the physiological heart rate of tree shrews. Conclusion We successfully established an ex vivo myocardial ischemia reperfusion model using tree shrews, and ischemia for 30 min followed by reperfusion for 30 min is the optimal experimental setting.关键词
心肌缺血再灌注/离体动物模型/实验树鼩Key words
myocardial ischemia reperfusion/model in vitro/tree shrews引用本文复制引用
杨天睿,苗云波,张荣平,余锦雯..实验树鼩心肌缺血再灌注离体模型的构建[J].南方医科大学学报,2018,38(2):205-210,6.基金项目
云南省内设研究机构云南省老年病防治研究中心2016年立项课(2016NS204) （2016NS204）
云南省卫计委医学后备人才培养计划(H-201615) （H-201615）
云南省应用基础研究(昆医联合专项)(2017FE468-112)云南省教育厅科学研究基金一般项目(2015Y051) （昆医联合专项）
