南方医科大学学报2018，Vol.38Issue(2)：211-216,6.DOI:10.3969/j.issn.1673-4254.2018.02.15
ADS-J1对SEVI增强HIV-1初始传播病毒及其慢性控制病毒感染的拮抗作用及机制
ADS-J1 antagonizes semen-derived enhancer of virus infection-mediated enhancement of transmitted founder HIV-1 and its matched chronic control strain infection
摘要
Abstract
Objective To investigate the effect of semen-derived enhancer of virus infection (SEVI) on the infection of transmitted/founder (TF) HIV-1 and its matched chronic control (CC) viruses and the antagonism of ADS-J1 on SEVI-mediated enhancement of TF and CC virus infection in vitro. Methods PAP248-286 self-assembling into SEVI amyloid fibrils was validated by ThT assay. We generated the virus stocks of TF and CC virus pair. TZM-bl cells were infected with the mixture of SEVI and TF or CC viruses for 72 h. Luciferase activity was used to observe the enhancement of SEVI. SEVI was treated with different concentrations of ADS-J1 and incubated with TF or CC viruses. TZM-bl cells were then infected with the mixture and luciferase activity was detected 72 h after infection to analyze the antagonism of ADS-J1 on the enhancing effect of SEVI. ADS-J1 was also incubated with TF and CC viruses directly and TZM-bl cells were infected for 72 h to evaluate the antiviral effect using luciferase assay. SEVI was treated with ADS-J1 and Zeta potential was determined to explore the antagonistic mechanism of ADS-J1. Results ThT assay showed that PAP248-286 was capable of self-assembly into SEVI amyloid fibrils. SEVI significantly accelerated TF and CC viruses infection (P<0.05), and ADS-J1 not only significantly antagonized the enhancement of SEVI (P<0.05) but also directly inhibited the infection of TF and CC viruses (P<0.05). ADS-J1 neutralized the positive charge of SEVI in a dose-dependent manner. Conclusions SEVI promotes the infection of TF and CC strains, and ADS-J1 antagonizes SEVI-mediated enhancement of TF and CC viruses by neutralizing the positive charge of SEVI.关键词
精液源性病毒增强因子/初始传播病毒/慢性控制病毒/感染/ADS-J1Key words
semen-derived enhancer of virus infection/transmitted founder virus/chronic control virus/infection/ADS-J1引用本文复制引用
柳红妙,麻宁宁,罗春,袁淑英,刘付励,姚新刚,周春琼,邹敏..ADS-J1对SEVI增强HIV-1初始传播病毒及其慢性控制病毒感染的拮抗作用及机制[J].南方医科大学学报,2018,38(2):211-216,6.基金项目
广东省卫生厅医学科研基金(B2013233) （B2013233）
南方医科大学科研启动计划(B1012021) （B1012021）
