南方医科大学学报2018，Vol.38Issue(2)：217-223,7.DOI:10.3969/j.issn.1673-4254.2018.02.16
聚乳酸-羟基乙酸共聚物载肝细胞生长因子纳米粒的构建及生物学活性评价
Construction and bioactivity evaluation of hepatocyte growth factor-loaded poly (lactic-co-glycolic acid) nanoparticles
摘要
Abstract
Objective To explore the optimum conditions for preparing poly(lactic-co-glycolic) acid (PLGA) nanoparticles and evaluate the bioactivity of hepatocyte growth factor (HGF)-loaded PLGA nanoparticles. Methods Bovine serum albumin (BSA)-loaded PLGA nanoparticles were prepared using a double emulsion-solvent evaporation method. The preparation process of nanoparticles was optimized by orthogonal test with the particle size, encapsulation efficiency (EE), drug loading (DD), and recovery as the indexes. HGF-loaded nanoparticles were then prepared under the optimized conditions. The EE, DD and release characteristics of BSA-loaded nanoparticles and HGF-loaded nanoparticles were evaluated using a BCA kit and HGF ELISA kit. The bioactivity of HGF-loaded nanoparticles was evaluated using CCK8 proliferation assay. Results The HGF-loaded nanoparticles prepared under the optimized conditions had a uniform size with a mean diameter of 234.4±4.8 nm, an EE of (77.75±3.04)％and a recovery rate of (49.33±9.34)％. The in vitro release curve highlighted an initial burst drug release followed by sustained release from the nanoparticles. HGF-loaded nanoparticles obviously promoted the proliferation of Hacat keratinocytes in vitro. Conclusion HGF-loaded nanoparticles prepared using double emulsion-solvent evaporation method under optimized conditions possesses a high EE with a good sustained drug release profile and a good bioactivity.关键词
聚乳酸-羟基乙酸共聚物纳米粒/正交试验/肝细胞生长因子/缓释Key words
poly (lactic-co-glycolic acid)/nanoparticles/orthogonal test/hepatocyte growth factor引用本文复制引用
冼文娇,王雪儿,张琳..聚乳酸-羟基乙酸共聚物载肝细胞生长因子纳米粒的构建及生物学活性评价[J].南方医科大学学报,2018,38(2):217-223,7.基金项目
广东省"珠江学者"特聘教授(GDUPS2013) （GDUPS2013）
广东省自然科学基金研究团队项目(2014A030312013) （2014A030312013）
广州市科技计划项目(201604020002) （201604020002）
