干旱地区农业研究2017，Vol.35Issue(6)：116-123,8.DOI:10.7606/j.issn.1000-7601.2017.06.18
5-氮杂胞苷对低温胁迫下黄瓜幼苗光合作用的影响
Effect of 5-azacytidine on photosynthesis of cucumber seedlings under low temperature and light intensity
摘要
Abstract
The objective of this study was to explore the effect of reduction in methylation level of genomic DNA on photosynthesis of cucumber seedlings under low temperature.'Jinyan No.4'cucumber seedlings was used as the experi-mental materials,and the effects of different concentrations of 5-azaC on the leaf gas exchange parameters,chlorophyll fluorescence quenching and cell membrane permeability of cucumber seedlings under low temperature(10℃/6℃,300 μmol·m-2·s-1)were studied.The results showed that the net photosynthetic rate(Pn),stomatal conductance(Gs), transpiration rate(Tr),the maximum photochemical efficiency of PSⅡ(Fv/Fm),the actual photochemical efficiency (ΦPSⅡ),the photosynthetic electron transport efficiency(ETR)and the photochemical quenching coefficient(qP)of the cucumber seedlings leaf were increased,while leaf cell membrane permeability,intercellular CO2concentration (Ci),the non-photochemical quenching coefficient(NPQ)and the leaf photochemical quenching parameters[Y(NO)] were reduced by 500 to 800 mol·L-1of 5-azaC treatment under low temperature,thus improving the cold tolerance of cucumber seedlings.关键词
黄瓜幼苗/5-氮杂胞苷/低温/光合作用/耐冷性Key words
cucumber seedling/5-azaC/low temperature/photosynthesis/cold tolerance分类
农业科技引用本文复制引用
邢潇晨,索琳格,肖春燕,刘苗苗,刘慧英,崔金霞..5-氮杂胞苷对低温胁迫下黄瓜幼苗光合作用的影响[J].干旱地区农业研究,2017,35(6):116-123,8.基金项目
国家自然科学基金项目(31160404) （31160404）
石河子大学高层次人才科研启动资金专项(RCZX201213) （RCZX201213）
