国际口腔医学杂志2017，Vol.44Issue(6)：654-659,6.DOI:10.7518/gjkq.2017.06.006
不同蔗糖浓度下外源性右旋糖酐酶与氟化钠对粘放线菌生物膜的影响
The influence of exogenous dextranase and sodium fluoride on biofilm of Actinomyces viscosus under different sucrose concentration conditions
摘要
Abstract
Objective To explore the impact of exogenous dextranase(Dex) and sodium fluoride(NaF) on the biomass,biofilm vitality and acid production of Actinomyces viscosus in different sucrose concentration substrate.Methods Crystal violet staining was used to quantify the biomass of A.viscosus in each group.2,3 bis(2 methoxy 4 nitro 5 sulfophenyl) 5 [(phenylamino)carbonyl] 2H tetrazolium hydroxide(XTT) assay was used to detect the vitality of the bacterial biofilms.pH electrode was applied to measure the pH values of the initial(pH1) and the 18 h liquid culture medium(pH2),and then the △pH(pH1-pH2) values was calculated.Results Under different sucrose concentration conditions,the bacterial biomass increased with the increase of sucrose concentration,and reached the peak at a sucrose mass fraction of 0.5％,and then decreased with the increase of sucrose concentration.In the 1％ sucrose mass fraction groups,the bacterial biomass formed in the Dex+NaF group was significantly less than in the Dex or NaF group.In all groups,the bacterial vitality was decreased along with the increased sucrose concentration.Under the same sucrose concentration,the bacteria vitality of the Dex group was similar to that of the control group(P＞0.05),and there was a slight increase in the bacteria vitality in the NaF group compared with the control group(P＜0.05).With the increase of sucrose concentration,△pH changes were first increased and then decreased.The single or combined use of NaF and Dex could inhibit the acid production of A.viscosus under the sucrose mass fraction ranged from 0.25％ to 2％.Conclusion Sucrose concentration was one of the factors that can influence the effect of Dex and NaF,both single and combination,on the bacterial biomass,biofilm vitality and acid production.The inhibitory effect of the combined use of Dex and NaF on the formation of bacterial biofilm showed a synergistic effect at the sucrose mass fraction of 1％.Dex and NaF displayed synergistic effect on acid production at the sucrose mass fraction of 2％.关键词
右旋糖酐酶/氟化物/生物膜/蔗糖/粘放线菌Key words
dextranase/sodium fluoride/biofilm/sucrose/Actinomyces viscosus分类
医药卫生引用本文复制引用
房宏志,田媛媛,喻譞,杨英明,杨惠,胡涛..不同蔗糖浓度下外源性右旋糖酐酶与氟化钠对粘放线菌生物膜的影响[J].国际口腔医学杂志,2017,44(6):654-659,6.基金项目
国家自然科学基金(81400507) （81400507）
四川省科技厅应用基础研究项目(2013JY0164)This study was supported by National Natural Science Foundation of China(81400507) and Applied Basic Re-search Project of Sichuan Provincial Science and Technology Department(2013JY0164). （2013JY0164）
