国际口腔医学杂志2017，Vol.44Issue(6)：679-685,7.DOI:10.7518/gjkq.2017.06.011
机械牵张力促进小鼠骨髓间充质干细胞的成骨向分化
Mechanical strain induces mouse bone mesenchymal stem cells osteogenic differentiation
摘要
Abstract
Objective This study aimed to investigate the stimulating mechanism of p38 mitogen-activated protein kinase(MAPK) signaling pathway and Osterix under intermittent mechanical stretch tension to accelerate the osteogenic differentiation of bone mesenchymal stem cells(BMSCs) in mice.Methods Three groups of C57BL/6J BMSC,namely,blank control group,strain group,and inhibitor group(p38MAPK signal pathway inhibitor SB203580 + strain),were prepared and exposed to 0.8％ intermittent mechanical strain at 0.5 Hz twice a day for 30 min at each time by using a Flexercell strain unit.The cells in the three groups were then harvested on days 1,3,and 5,respectively.Changes in the mRNA expression of ALP,COL Ⅰ,and OCN were detected through real time-polymerase chain reaction(RT-PCR) and the protein expression of P-p38MAPK was observed through Western blot analysis.osterix gene was knocked down by small interfering RNA(siRNA),and Osterix protein expression was determined through Western blot.The mRNA expression levels of ALP,COL Ⅰ,and OCN were identified through RT-PCR.Results Mechanical tension force could promote the mRNA expression of ALP,COL Ⅰ,OCN,and osterix.osterix silencing could decrease the mRNA expression of ALP,COL Ⅰ,and OCN.Western blot revealed that the protein expression levels of Osterix and P-p38MAPK in the strain group were significantly higher than those in the control group(P＜0.05).The mRNA expression of ALP,COL Ⅰ,OCN,and osterix decreased after SB203580 was used.Conclusion Intermittent mechanical strain promotes the osteogenic differentiation of BMSC via the p38MAPK-Osterix pathways.关键词
间断性牵张力/骨髓间充质干细胞/成骨向分化/p38MAPK信号通路Key words
intermittent stretching force/bone marrow stem cell/osteoblastic differentiation/p38MAPK signal pathway分类
生物科学引用本文复制引用
薛令法,张岱尊,肖文林,于保军..机械牵张力促进小鼠骨髓间充质干细胞的成骨向分化[J].国际口腔医学杂志,2017,44(6):679-685,7.基金项目
山东省高校科技计划(J12LK57) （J12LK57）
青岛市黄岛区应用研究与公共卫生专项基金(2014-1-84)This study supported by Science and Technology Program in Colleges and Universities of Shandong(J12LK57) and Special Fund for Applied Study and Public Health,Huangdao District,Qingdao(2014-1-84). （2014-1-84）
