国际病理科学与临床杂志2017，Vol.37Issue(10)：2013-2030,18.DOI:10.3978/j.issn.2095-6959.2017.10.001
利用蛋白质组学筛选肾透明细胞癌肿瘤标志物
Potential tumor markers of renal clear cell carcinoma with proteomic
摘要
Abstract
Objective:To identify and validate the potential tumor markers of renal clear cell carcinoma for progress assessment and clinical treatment with proteomic.Methods:We analyzed 16 cases of tumor and pericarcinomatous tissue with two-dimensional difference gel electrophoresis (2D-DIGE) and Q-Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS),and then identified the abnormal expression protein with Western blot,Real-time quantitative PCR and IHC.Results:A total of 1 873 proteins were quantified with proteomic in 16 cases of renal clear cell carcinoma,and 51 proteins of 68 sports that more than 1.5-fold differential expressing were identified,including 16 proteins upregulated and 35 proteins downregulated in renal cell carcinoma (RCC) (P＜0.05).Among them,the up-expressed NNMT,Annexin Ⅳ and down-expressed ACY1,calbindin in DIGE were confirmed with 16 cases of tumor and pericarcinomatous tissues with qRT-PCR and Western blot (P＜0.01).Subsequently,SPSS analysis ofIHC validated ACY1 decreased in 53 cases of clear cell renal cell carcinoma (P＜0.001),and Annexin Ⅳ increased in 47 cases (P＜0.001).Conclusion:ACY1 and Annexin Ⅳ serve as a putative tumor suppressor and a potential treatment marker for clear cell RCC.关键词
肾透明细胞癌/肿瘤标志物/蛋白质组学/二维差异凝胶电泳Key words
clear cell renal cell carcinoma/tumor marker/proteomics/two-dimensional difference gel electrophoresis引用本文复制引用
郭丹,王安琪,赵晓晓,刘辉,孙健..利用蛋白质组学筛选肾透明细胞癌肿瘤标志物[J].国际病理科学与临床杂志,2017,37(10):2013-2030,18.基金项目
国家科技重大专项(2011ZX09307-001-01) （2011ZX09307-001-01）
国家重点研发计划(2016YFC1201703).This work was supported by National Science and Technology Major Project Foundation (2011ZX09307-001-01) and the National Key Research and Development Program (2016YFC1201703),China. （2016YFC1201703）
