临床与病理杂志2017，Vol.37Issue(10)：2031-2037,7.DOI:10.3978/j.issn.2095-6959.2017.10.002
黄连素对多发性骨髓瘤细胞增殖和凋亡的影响
Effects of berberine on proliferation and apoptosis of multiple myeloma cells
摘要
Abstract
Objective:To investigate the effect of berberine on the activity and apoptosis of multiple myeloma cells.Methods:The proliferation of multiple myeloma U266 cells under different concentrations of berberine were detected by CCK-8.ELISA was used to detect the secretion of IL-6 by U266 under different concentrations of berberine.The apoptosis of U266 myeloma cells was detected by flow cytometry under different concentrations of berberine.The ultrastructure was observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).Results:Treatment of U266 cells with berberine (40-160 μmol/L) suppressed cell proliferation and IL-6 secretion in dose-and time-dependent manners.At the same time,berberine dose-dependently induced G2/M phase arrest and apoptosis in U266 cells.The ultrastructure ofU266 cells treated with 80 μmol/L berberine was morphologically characterized by apoptosis.Conclusion:Berberine can inhibit the activity of human multiple myeloma cells,induce G2/M stagnation and induce apoptosis.关键词
黄连素/多发性骨髓瘤/凋亡/细胞增殖Key words
berberine/multiple myeloma/apoptosis/cell proliferation引用本文复制引用
李敬东,韩效林,杨翠,孙玲..黄连素对多发性骨髓瘤细胞增殖和凋亡的影响[J].临床与病理杂志,2017,37(10):2031-2037,7.基金项目
河南省医学科技公关计划项目(201203068). This work was supported by Henan Medical Science and Technology Public Relations Project,China (201203068). （201203068）
