临床与病理杂志2017，Vol.37Issue(10)：2043-2049,7.DOI:10.3978/j.issn.2095-6959.2017.10.004
Carba NP直接纸片法检测肠杆菌科细菌碳青霉烯酶的应用评估
Clinical evaluation of Carba NP paper strip test in detection of carbapenemase-producing Enterobacteriaceae
摘要
Abstract
Objective:To evaluate Carba NP paper strip test for the carbapenemase detection of carbapenem-resistant Enterobacteriaceae.Methods:A total of 122 Enterobacteriaceae were isolated from various clinical samples from the Second People's Hospital between April 1,2015 and December 31,2016.The phenotype of Enterobacteriaceae were detected by Carba NP test and Carba NP paper strip test,the genes of carbapenemase detection were performed by PCR,which was regarded as a gold standard method to gene detection.Results:A total of 112 blaKPC-2 isolates,8 blaNDM-1 isolates,no class D carbapenemase isolate,and 2 non-carbapenemase isolates were detected in 122 carbapenem-resistant Enterobacteriaceae.The sensitivity of Carba NP test and Carba NP direct paper to the carbapenemase of the tested strain was as high as 98％ and the specificity was as high as 100％,refer to PCR results.Carba NP test can be done within one hour,and Carba NP paper strip test can be done within five minutes.Conclusion:Relative to the Carba NP test,Carba NP simple paper method does not need expensive protein extraction liquid,cheap and easy to operate,suitable for primary hospitals are widely used,which can effectively assist clinical anti-infection treatment.关键词
Carba NP直接纸片试验/碳青霉烯酶/检测价值Key words
Carba NP paper strip test/carbapenemase/detection value引用本文复制引用
胡锡池,胡仁静,刘小云..Carba NP直接纸片法检测肠杆菌科细菌碳青霉烯酶的应用评估[J].临床与病理杂志,2017,37(10):2043-2049,7.基金项目
无锡市科技支撑项目(CSE31N1602).This work was supported by Science and Technology Bureau of Wuxi,China (CSE31N1602). （CSE31N1602）
