海南医学院学报2017，Vol.23Issue(21)：2906-2909,4.DOI:10.13210/j.cnki.jhmu.20171027.001
健脾补肾方剂含药血清对结肠癌细胞生长及血管新生的影响
Effect of the Jianpibushen Prescription-containing serum on the colon cancer cell growth and angiogenesis
摘要
Abstract
Objective:To study the effect of Jianpibushen Prescription-containing serum on the colon cancer cell growth and angiogenesis.Methods:SD male rats were taken and given intragastric administration of Jianpibushen Prescription,and arterial blood was collected after 1 week to prepare drug-containing serum;colon cancer cell line HCT116 was cultured and divided into two groups.The intervention group was treated with DMEM containing 10％ drug-containing serum,and the control group was treated with DMEM containing 10％ drug-free serum.After 24 hours of treatment,the mRNA expression of proliferation genes,invasion genes and angiogenesis genes in cells were measured.Results:EZH2,USP22,Nanog,FAK,CDC37,HOXB9,TROP2,ELMO3,c met,HIF-1α,VEGF and Ang 2 mRNA expression in intervention group of colon cancer cells were significantly lower than those in control group while ASPP2,MS4A12,TWEAK and Fn14 mRNA expression were significantly higher than those in control group.Conclusion:Jianpibushen Prescription-containing serum has inhibitory effect on the colon cancer cell proliferation and invasion as well as angiogenesis.关键词
结肠癌/健脾补肾方剂/增殖/侵袭/血管新生Key words
colon cancer/Jianpibushen Prescription/proliferation/invasion/angiogenesis分类
医药卫生引用本文复制引用
王元惠,张盛倢,胡晓颖,周振华..健脾补肾方剂含药血清对结肠癌细胞生长及血管新生的影响[J].海南医学院学报,2017,23(21):2906-2909,4.基金项目
上海市卫生和计划生育委员会中医药科研专项(2016JP005)the Shanghai municipal health and Family Planning Committee of Chinese medicine research project (2016JP005) （2016JP005）
