化工学报2018，Vol.69Issue(1)：341-351,11.DOI:10.11949/j.issn.0438-1157.20171040
抗体药物分离纯化中的层析技术及进展
Chromatographic technology in antibody purification and its progress
摘要
Abstract
Antibody, especially monoclonal antibody has become an important therapeutic drug which has a broad market demand and prospect.Nowadays the bottleneck of antibody production has shifted from antibody expression to downstream processing.The typical antibody purification process starts with protein A affinity chromatographic to capture the antibody and includes two subsequent polishing steps for impurity removal, such as ionic exchange, hydrophobic interaction or hydroxyapatite chromatography.There are some limitations restricting the development in antibody industry.Therefore, the researchers are committed to develop novel purification methods, including mixed-mode chromatography (MMC) and short peptide biomimetic chromatography which remedy the weakness of traditional methods.According to the recent advances in the purification of monoclonal antibody drugs, protein A affinity chromatography and the novel chromatographic methods and their applications in monoclonal antibody purification are reviewed in the present article.关键词
抗体药物/单克隆抗体/纯化/层析/混合模式/短肽仿生/分子模拟Key words
antibody drug/monoclonal antibody/purification/chromatography/mixed-mode/short peptide biomimetic/molecular simulation分类
化学化工引用本文复制引用
卢慧丽,林东强,姚善泾..抗体药物分离纯化中的层析技术及进展[J].化工学报,2018,69(1):341-351,11.基金项目
国家自然科学基金项目(21576233,21476198) （21576233,21476198）
中国博士后科学基金项目(512100-X91603). supported by the National Natural Science Foundation of China(21576233,21476198). （512100-X91603）
