华南农业大学学报2018，Vol.39Issue(2)：29-33,5.DOI:10.7671/j.issn.1001-411X.2018.02.005
碳离子束辐照水稻诱变效应及突变体的筛选
Mutagenic effects of carbon ion beam irradiation on rice and screening for induced mutants
摘要
Abstract
[Objective]To enrich rice (Oryza sativa L.) mutant library, rapidly create genetically stable new germplasm,and provide valuable materials for following study of mutation mechanisms and rice genetic breeding.[Method]The breeder seeds of 'IR55', 'IR60', 'Sanlicun' and 'Yunnan Ludao' were irradiated by carbon ion beam, and the agronomic traits of induced generations were consecutively investigated.[Result]The mutants of four cultivars varied over a large range of variation in plant height, flag leaf length, spikelet length, length-width ratio of grain and other traits, and displayed obvious positive or negative mutation. Seed setting rate decreased respectively with the lowest value of 2％. Large genetic diversity variations were detected between the mutants and breeder seed populations of four cultivars using randomly selected SSR markers, indicating that heavy ion irradiation induced big fragment variations at DNA sequences, rather than subtle site mutation.[Conclusion]Carbon ion beam irradiation can cause positive or negative mutation of plant height, flag leaf length, grain shape and other agronomic traits of rice. New rice germplasms with variation in agronomic traits can be screened out from mutation progenies.关键词
水稻/碳离子束辐照/诱变效应/突变体Key words
rice (Oryza sativa L.)/carbon ion irradiation/mutagenic effect/mutant分类
农业科技引用本文复制引用
杨瑰丽,陈莹,郭涛,黄明,黄翠红,王慧,李文建,陈志强..碳离子束辐照水稻诱变效应及突变体的筛选[J].华南农业大学学报,2018,39(2):29-33,5.基金项目
国家重点研发计划专项资金(2016YFD0102102) （2016YFD0102102）
现代农业产业技术体系建设专项资金(CARS-01-12) （CARS-01-12）
广东省应用型研发专项资金(2015B020231011) （2015B020231011）
