华南农业大学学报2018，Vol.39Issue(2)：40-46,7.DOI:10.7671/j.issn.1001-411X.2018.02.007
不同品种果蔗幼苗对低温的生理响应及耐寒性评价
Physiological response and cold resistance evaluation of seedlings of different chewing cane cultivars to low temperature
摘要
Abstract
[Objective]Low temperature is the main limiting factor affecting cultivation of chewing cane (Saccharum officinarum L.) in north China. To investigate the physiological mechanisms of low temperature adaptation and physiological responses of chewing cane cultivars under low temperature stress,and provide a theoretical basis for chewing cane cold resistance germplasm screening and breed improvement.[Method]Six chewing cane cultivars seedlings were used as test materials to determinate the effect of low temperature stress on the seedlings using the indexes such as relative conductivity(REC), malondialdehyde (MDA), soluble sugar (SS), soluble protein (SP) and proline (Pro) contents and superoxide dismutase (SOD), peroxidase (POD),catalase (CAT) and ascorbate peroxidase(APX) activities in artificial simulation low temperature environment. Taking cold resistant coefficient of each physiological index of chewing cane leaves as the index to measure cold resistance capacity, and cold resistance of chewing cane cultivars were comprehensively evaluated using correlation analysis, principal component analysis and membership function analysis based on cold resistant coefficients of these indexes. Evaluations of cold tolerance of six chewing cane cultivars seedlings were made through fuzzy membership function analysis.[Result]With the extension of low temperature stress time, the REC, SS, SP and Pro contents of six chewing cane cultivar seedling leaves kept the overall rising trend, and the MDA content, the activities of SOD, POD, CAT and APX showed the trend of increasing at first and then decreasing. The cold tolerance order of comprehensive evaluation was 'Guiguozhe1'>'Badila'='Minyinhuangpiguozhe'>'Baiyuguozhe'>'Chuanzhe26'>'Longheiguozhe'.[Conclusion]Membership function analysis is simple and accurate, which can be used as an effective method to identify cold hardiness of chewing cane. The adaptabilities of different chewing cane cultivars to long lasting low temperature are different. The cold resistance of chewing cane is not only related to its own genetic factors and physiological characteristics, but also closely related to the external environmental factors.关键词
果蔗/抗寒性/生理指标/综合评价Key words
chewing cane/cold resistance/physiological index/comprehensive evaluation分类
农业科技引用本文复制引用
陈明辉,程世平,张志录,张保青..不同品种果蔗幼苗对低温的生理响应及耐寒性评价[J].华南农业大学学报,2018,39(2):40-46,7.基金项目
国家自然科学基金(31760415) （31760415）
河南省科技厅科技攻关计划项目(152102110007) （152102110007）
河南省教育厅重点科研项目(16A220004) （16A220004）
广西农业科学院科技发展基金(2015JZ01) （2015JZ01）
广西重点实验室建设项目(16-A-01-01) （16-A-01-01）
