华南农业大学学报2018，Vol.39Issue(2)：95-102,8.DOI:10.7671/j.issn.1001-411X.2018.02.015
产酸、耐酸乳酸菌的分离鉴定及益生特性
Isolation, identification and probiotic characteristics of acid- producing and acid-resistant Lactobacillus strains
摘要
Abstract
[Objective]To isolate, screen and evaluate Lactobacillus strains with excellent acid-producing ability, and offer materials for application of Lactobacillus in feed industry.[Method]Using acid flavor food as samples, we isolated and screened Lactobacillus strains, initially according to the size of calcium dissolving zone and subsequently according to acid tolerance. The acid-producing abilities, growth characteristics, low-pH and bile salt tolerance, and antibacterial activities of Lactobacillus strains screened out were further studied. [Result]Ten Lactobacillus strains capable of growing at pH 3.0 were obtained. Strain SC3A and DJ3 were identified as L. pentosus, and strain SC15, SC16, DJ9B, DJ10C, SC8, DJ8A, DJ8B and DJ9A were identified as L. fermentum. The acid-producing abilities, fermentation growth rate and antibacterial activities of L. pentosus strains DJ3 and SC3A were significantly superior to those of the L. fermentum stains. The L. fermentum strains DJ8A and DJ9B showed higher tolerance to acid environment and bile salt.[Conclusion]Ten Lactobacillus strains obtained from acid flavor food have strong acid-producing ability, acid tolerance and antibacterial activity, and have application potential in food and feed industries.关键词
乳酸菌/分离鉴定/产酸/抑菌活性/耐酸/耐胆盐Key words
Lactobacillus/isolation and identification/acid production/antibacterial activity/acid tolerance/bile salt tolerance分类
农业科技引用本文复制引用
林龙镇,邹卫玲,李安章,朱红惠..产酸、耐酸乳酸菌的分离鉴定及益生特性[J].华南农业大学学报,2018,39(2):95-102,8.基金项目
广东省科技创新领军人才项目(2015TX01N036) （2015TX01N036）
广东省科学院科研平台环境与能力建设专项(2016GDASPT-0302) （2016GDASPT-0302）
广东省科学院实施创新驱动发展能力建设专项(2017GDASCX-0402) （2017GDASCX-0402）
