湖南农业大学学报（自然科学版）2018，Vol.44Issue(1)：39-44,6.DOI:10.13331/j.cnki.jhau.2018.01.008
DSM-18020菌株expI基因的克隆及生物信息学分析
Cloning and bioinformatics analysis of expI gene from strain DSM-18020
摘要
Abstract
In order to study the signal molecule synthase gene expI of Dickeya dadantii, the gene expI was amplified from the genome of the type strain DSM-18020. Bioinformatics analysis showed that there was a complete open reading frame (ORF) in DSM-18020 expI, which was 639 bp in length and had 99％ homology to N-acyl-homoserine lactone (AHLs) synthetase of D. dadantii 3937. The molecular weight of the protein encoded by DSM-18020 expI is 24,704, and the theoretical isoelectric point is 5.85. The hydrophobic amino acids are more abundant than hydrophilic amino acids and distributed evenly throughout the peptide chain, with no signal peptide. expI gene was cloned into the expression vector pET28α and transformed into Escherichia coli BL21 (DE3). The expression product was basically identical with the expectation.关键词
迪基氏菌/DSM-18020菌株/群体信号分子合成酶基因expI/生物信息学Key words
Dickeya/DSM-18020 strain/signal molecular synthetase gene expI/bioinformatics分类
生物科学引用本文复制引用
程毅,周静煌,严准,高春生,李智敏,余永廷..DSM-18020菌株expI基因的克隆及生物信息学分析[J].湖南农业大学学报（自然科学版）,2018,44(1):39-44,6.基金项目
中国农业科学院科技创新工程项目(CAAS-ASTIP-2015-IBFC09) （CAAS-ASTIP-2015-IBFC09）
湖南省自然科学基金项目(2017JJ3351) （2017JJ3351）
中央级公益性科研院所基本科研业务费专项(1610242016025) （1610242016025）
