湖南农业大学学报（自然科学版）2018，Vol.44Issue(1)：77-81,5.DOI:10.13331/j.cnki.jhau.2018.01.015
牛传染性鼻气管炎病毒gD蛋白的原核表达及其免疫原性
Expression and immunogenicity of protein gD from virus in infectious rhinotracheitis of bovine
丁国伟 1李琛 1李玉安 1叶正琴 1宋庆庆1
作者信息
- 1. 扬州优邦生物药品有限公司,江苏 扬州 225008
- 折叠
摘要
Abstract
The expression and immunogenicity of conserved domain of protein gD were researched for developing antibody detection kit of IBRV virus. Specific primers were designed for the amplification of the gene gD sequence with the immunoglobulin-like domain based on the full length gene gD sequence from bovine herpesvirus, type 1 (GenBank ID NO. NC_001847). The immunoglobulin-like domain gene of gD protein was inserted into plasmid pET-32a after PCR amplication, and was named as pET32a-ΔgD. The expression vector was then transferred into strain E. coli Rosseta (DE3) with aim to induce the recombinant protein. The result showed that 43000 recombinant proteins were obtained through the analysis of SDS-PAGE, and the relative molecular mass of them were accordance with the theoretical value of recombinant protein gD. After purified from nickel column, the concentration of them was 2.5 mg/mL, with the purity of about 95％. The recombinant proteins were proved of good immunogenicity after experienced the identification of Western Blot and Elisa, which indicated that they could be used as antigen for the development of antibody detection kit of IBR.关键词
牛传染性鼻气管炎病毒(IBRV)/gD蛋白/原核表达/免疫原性Key words
IBRV/gD protein/prokaryotic expression/immunogenicity分类
农业科技引用本文复制引用
丁国伟,李琛,李玉安,叶正琴,宋庆庆..牛传染性鼻气管炎病毒gD蛋白的原核表达及其免疫原性[J].湖南农业大学学报（自然科学版）,2018,44(1):77-81,5.
