海洋科学2017，Vol.41Issue(9)：1-8,8.DOI:10.11759/hykx20160414001
基于三种分子标记的外伶仃岛石珊瑚系统进化关系研究
Study of phylogenetic relationships of scleractinian from Wail-ingding Island based on three standard molecular markers
摘要
Abstract
The mitochondrial cytochrome oxidase subunit I (CO I), 16S rRNA, and nuclear ITS genes have been routinely used as standard molecular markers. A total of five species pertaining to four genera of scleractinian, col-lected from Wailingding Island, Zhuhai City, Guangdong Province, were analyzed by partial sequences, and the genetic distances of these three genes were calculated. The results indicated that the average AT contents of CO I and 16S rRNA gene fragments were obviously higher than the GC contents. However, the average AT content of ITS fragments was obviously less than the GC content. The average genetic distances of the three genes were 0.043, 0.134, and 0.763 from the five species, respectively. The neighbor-joining, maximum-likelihood, and mini-mum-evolution trees of combined partial sequences of these three genes revealed that the phylogenetic relationships were consistent with the morphological classification in the families. However, the phylogenetic relationships were slightly different from the morphological classification in Faviidae. This result suggested that morphological classi-fication may be limited by coral skeleton plasticity. These findings provide basic data for studying the genetic rela-tionship of Wailingding Island corals and a theoretical basis for resource protection of stone corals in this area.关键词
石珊瑚/COI/16S rRNA/ITS/分子系统进化Key words
Scleractinian/COI/16S RNA/ITS/Molecular phylogenetics分类
生物科学引用本文复制引用
肖宝华,廖宝林,杨小东,谢子强..基于三种分子标记的外伶仃岛石珊瑚系统进化关系研究[J].海洋科学,2017,41(9):1-8,8.基金项目
广东省渔港建设和渔业产业发展专项(A201708D06) （A201708D06）
大鹏新区产业发展专项(KY20160107) （KY20160107）
Guangdong Ocean and Fishery Administration Project, No. A201708D06 （）
Dapeng New District Industry Development Fund, No. KY20160107 （）
