解放军医学杂志2017，Vol.42Issue(12)：1061-1065,5.DOI:10.11855/j.issn.0577-7402.2017.12.07
黄芪神仙定年方对辐射小鼠肝氧化应激损伤的防护作用
Protective effects of Astragalus immortal prescription against liver oxidative stress in irradiated mice
摘要
Abstract
Objective To explore the protective effect and possible mechanism of Astragalus immortal prescription (AIP) from hepatic oxidative stress in mice irradiated by X ray.Methods Six to eight weeks old Kunming mice were randomly divided into normal control group,irradiation alone group,positive control group,and low,medium and high dose groups of AIP (10 rats in each group),physiological saline,thymus peptide and different concentration decocta of AIP were given respectively.After continuous gavage for 10 days,the whole body was irradiated with X ray at the dose of 8 Gy at a time,and then continued lavaging for 3 days,with weighing the mice and observing the diet,water intake and general condition.After killing the mice,the liver was take and the liver index was calculated;the morphological changes of the liver tissues were observed and the expressions of superoxide dismutase (SOD),malondiadehyde (MDA),glutathione peroxidase (GSH-Px) and nuclear factor erythroid 2-related factor 2 (Nrf2) in the liver tissues were detected.Results Compared with the normal control group,the weight of mice in model group decreased obviously (P＜0.01);pathological section showed that the liver cells were disordered,the central vein was congested and blocked seriously,the surrounding liver cells were denatured and necrotic;the activity of SOD and GSH-Px in liver tissues decreased,and the content of MDA increased (P＜0.05),the expression of Nrf2 protein increased significantly (P＜0.01).Compared with the radiation alone group,the weight of mice in positive control group and high dose AIP group increased significantly (P＜0.01);pathological section showed that the liver tissue structure was basically normal,the liver cells were radially arranged with the central vein as the center;the activity of SOD and GSH-Px in liver tissues increased,and the content of MDA decreased (P＜0.05),the expression of Nrf2 protein decreased significantly (P＜0.01).Conclusion AIP has protective effect from hepatic oxidative stress in mice irradiated by X ray,and the mechanism may be related to its antioxidant effects.关键词
黄芪神仙定年方/辐射/肝脏/氧化应激Key words
Astragatus immortal prescription/radiation/hepatic/oxidative stress分类
医药卫生引用本文复制引用
冯彩琴,刘永琦,张利英,辛晓明,张同同,李娟,张苡铭,陈刚,张丽昕..黄芪神仙定年方对辐射小鼠肝氧化应激损伤的防护作用[J].解放军医学杂志,2017,42(12):1061-1065,5.基金项目
甘肃省重大疾病分子医学与中医药防护重点实验室开放基金(FZYX14-10) （FZYX14-10）
敦煌医学与转化省部共建教育部重点实验室开放课题(DHYX14-011) （DHYX14-011）
This work was supported by the Fund of the Key Laboratory of Ministry of Education for Dunhuang Medicine and Transformation (DHYX14-011),and Open Fund of Key Laboratory of Molecular Medicine and Traditional Chinese Medicine Protection of Major Diseases in Gansu Province (FZYX14-10) （DHYX14-011）
