解放军医学杂志2017，Vol.42Issue(12)：1066-1071,6.DOI:10.11855/j.issn.0577-7402.2017.12.08
高血糖对大鼠局灶性脑缺血再灌注时小胶质细胞活化的影响
Effects of hyperglycemia on the activation of microglia in focal cerebral ischemia and reperfusion
摘要
Abstract
Objective To observe the changes of microglia in diabetic cerebral ischemia and reperfusion,and further explore the role of microglia in diabetic rats with cerebral ischemic injury.Methods Eighty healthy male SD rats were randomly divided into 4 groups:normal blood glucose sham operation group (NG sham group,n=5),diabetic hyperglycemia sham operation group (HG sham group,n=5),normal blood glucose with cerebral ischemia-reperfusion group (NG MCAO group,n=35) and diabetic hyperglycemia with cerebral ischemia-reperfusion group [HG middle cerebral artery occlusion (MCAO) group,n=35].The diabetic rats models were established by intraperitoneal injection of streptozotocin (STZ).The cerebral ischemia reperfusion models were made with MCAO,the specific marker protein Iba-1 was used to immunohistochemically label the microglia.The changes of microglia in the periventricular zone and caudate putamen region of the rats in HG MCAO group and NG MCAO group were observed at ischemia 30min and reperfusion 30min,3h,6h,Id,3d,7d and 14d (each time point contains 5 rats).Iba-1 and proliferating cell nuclear antigen (PCNA) immunofluorescence double labeling method were performed to detect the proliferation of microglia.Results After ischemia-reperfusion,the brain tissue appeared as obvious edema,mesh-like,HE staining faded,neurons swelled,cytoplasm vacuolization,nuclear pyknosis,and inflammatory cell infiltration.All these symptoms of brain injury were more obvious in HG group than in NG group.On the 3rd day after ischemia reperfusion,microglial cells were markedly activated in the infarct peripheral zone,piriform cortex and somatic sensory cortex,the activation reached the peak value at the 7th day,and the activated state continued to the 14th day of reperfusion.It was found with Iba-1 and PCNA immunofluorescence double labeling that,after cerebral ischemia-reperfusion,the increase of microglia number was related to its proliferation.The microglia proliferation also increased at the 3rd day after ischemia-reperfusion,and reached the peak value at the 7th day.The degree of microglia activation and proliferation was weaker in NG group than in HG group (P＜0.05),but higher obviously when compared with their each sham group (P＜0.05).Conclusion Hyperglycemia induced ischemia brain tissue microglia activation and proliferation inhibition may be involved in the hyperglycemia induced ischemic brain damage.关键词
小神经胶质细胞/高血糖/脑/再灌注损伤Key words
microglia/hyperglycemia/brain/reperfusion injury分类
医药卫生引用本文复制引用
褚冬,马艳梅,郭永真,张建忠,景丽..高血糖对大鼠局灶性脑缺血再灌注时小胶质细胞活化的影响[J].解放军医学杂志,2017,42(12):1066-1071,6.基金项目
宁夏医科大学校级课题(XY2017002) （XY2017002）
宁夏医科大学基础医学西部一流学科建设项目 （）
This work was supported by the School-Level Subject of Ningxia Medical University (XY2017002),and the Western First-Class Discipline Construction Projects of Ningxia Medical University Basic Medicine （XY2017002）
