检验医学与临床2018，Vol.15Issue(2)：151-153,156,4.DOI:10.3969/j.issn.1672-9455.2018.02.002
抗双链DNA抗体水平和抗体亲和力与SLE患者疾病状态的关系以及对 两种方法检测能力的影响
The correlation between the avidity or level of serum anti-dsDNA antibody with SLE disease progression and their influence on the performance of different detection techniques
摘要
Abstract
Objective To investigate the connection between the avidity or level of anti-dsDNA antibody with the progression of systemic lupus erythematosus (SLE) ,and their influence on the performance of two widely used techniques (ELISA and IIF ) .Methods Using commercially available kits based on ELISA and IIF techniques simultaneously ,serum anti-dsDNA antibody was determined from 300 patients with SLE ,495 patients with non-SLE autoimmune disease ,and 300 health controls (HCs) .Positive samples by ELISA were further determined for anti-dsDNA antibody avidity index using urea based ELISA kits .Results (1)the level and avidity index of anti-dsDNA antibody were significantly and insignificantly higher in SLE than non-SLE patients[antibody level :285 .8(164 .5-463 .3) IU/mL vs .172 .9(135 .3-200 .1) IU/mL ,P=0 .038 ;antibody avidity index :32 .3(19 .2 -50 .7)vs .15 .5(9 .5-49 .8) ,P=0 .169] ,and significantly correlated with SLEDAI scores ,respectively (P=0 .000 ,0 .002) .(2)For samples whose anti-dsDNA antibody were positive by both ELISA and IIF ,their anti-dsDNA antibody levels and avidity indices were significantly higher than those sole-ly positive by ELSIA(P<0 .05).Conclusion The closely correlation of anti-dsDNA level and avidity index with SLE disease progression ,signifies that antibody amount and avidity might have been involved in disease development ,and could also be used to monitor disease progression .For the determination of anti-dsDNA anti-body ,IIF was more likely to detect antibodies of relatively higher avidity ,in comparison with ELISA .关键词
抗双链DNA抗体/抗体亲和力/间接免疫荧光法/系统性红斑狼疮Key words
anti-dsDNA antibody/antibody avidity/indirect immunofluorescence/systemic lupus er-ythematosus分类
医药卫生引用本文复制引用
田野,刘晴,王晓宁,张国军,谭延国,张云聪,冯珍如,闫惠平,张岩,张海萍,李佩,郑芳芳,聂秋燕..抗双链DNA抗体水平和抗体亲和力与SLE患者疾病状态的关系以及对 两种方法检测能力的影响[J].检验医学与临床,2018,15(2):151-153,156,4.基金项目
国家卫生和计划生育委员会医药卫生科技发展研究中心专项课题资助(28-5-5) （28-5-5）
首都临床特色应用研究(吴阶平)基金资助项目(Z141107006614008). （吴阶平）
