南京师大学报（自然科学版）2017，Vol.40Issue(4)：109-115,7.DOI:10.3969/j.issn.1001-4616.2017.04.018
淡水驯化后大鳞鲃胚胎发育观察
Observation of Embryonic Development of Freshwater Adapted Barbus capito
摘要
Abstract
The fertilized eggs of Barbus capito were obtained by artificial insemination, and the morphological characteristic of embryonic development was observed in detail under the water temperature of 23 ℃ to 26 ℃. The results showed that the fertilized eggs were floating eggs(semi floating eggs),and the average egg diameter was(1.55± 0.1)mm. After absorbing water,the egg membrane was enlarged,and the diameter reached(4.3±0.2)mm. It took 47 h 29 min from fertilization to hatching-out with the average accumulative temperature of 1 081 ℃ to 1 222 ℃·h. The cleavage of fertilized eggs of B.capito was discoidal cleavage,like other cyprinid fishes. According to the morphological characteristics in all stages of the embryonic development process,it could be divided into 6 stages,including the uplift of blastoderm stage,cleavage stage,blastula stage,gastrula stage,neurula stage to tail-bud stage and embryonic twisting stage to hatching stage,and 22 developing phases. After entering the water,the unfertilized eggs absorbed water and swelled. Then,protoplasm centralized and formed"blastoderm",but no such as the 2 cell cleavage phenomenon. When the protuberance or blastomere of the unfertilized egg were leaving in succession,the"blastoderm"would form a"false blastocyst"like the fertilized egg in the late blastula stage. The results served the important guidance for artificial breeding and calculating fertility rate of B.capito.关键词
大鳞鲃/胚胎发育/未受精卵Key words
Barbus capito/embryonic development/unfertilized egg分类
农业科技引用本文复制引用
覃宝利,蔺玉华,丁辰龙..淡水驯化后大鳞鲃胚胎发育观察[J].南京师大学报（自然科学版）,2017,40(4):109-115,7.基金项目
江苏省水产三新工程(Y2016-15)、宿迁市农业科技自主创新(SQCX2016-04). （Y2016-15）
