水产学报2018，Vol.42Issue(2)：178-185,8.DOI:10.11964/jfc.20170510840
杂交三倍体泥鳅染色体C带及rDNA序列的FISH定位
Characterization of hybrid triploid loach (Misgurnus anguillicaudatus) karyotype by C-banding and fluorescence in situ hybridization with rDNA
摘要
Abstract
The embryonic chromosomes of hybrid triploid loach (4n♀×2n♂),Misgurnus anguillicaudatus, were examined by C-banding and fluorescence in situ hybridization (FISH).The characteristics of C-band of hybrid trip-loid loach were discussed for the first time, so as to provide the basis for identifying chromosomes accurately.Meanwhile, the number and position of ribosomal 5.8S+28S rDNA in the metaphase chromosomes of hybrid triploid loach embryos were studied.The results of C-banding in embryonic chromosomes of hybrid triploid loach show that, C-bands were classified into types of terminals and centromeres, but no middle type of arm was observed.The type of terminals were located on terminal of short arm of M1, whereas, compared with the type of centromeres, bigger and more obvious C-band was found on terminal of short arm of M1.While the other M chro-mosomes and SM chromosomes and T chromosomes only have centromeric C-band.The results of FISH show that, ribosomal 5.8S+28S rDNA was clearly localized in the region of terminal of middle centromere (M) of the metaphase chromosome of the hybrid triploid loach.Three hybrid signals could be detected in metaphase chromo-somes of hybrid triploid loach.The results further confirmed that the hybrid triploid loach is a genetic triploid with three sets of chromosomes from the aspect of molecular cytogenetics.关键词
泥鳅/杂交三倍体/C带/rDNA/FISHKey words
Misgurnus anguillicaudatus/hybrid triploid/chromosome banding/rDNA/fluorescence in situ hy-bridization (FISH)分类
生物科学引用本文复制引用
周贺,徐其征,高养春,张蕊,李霞,庄子昕,梁雨婷,李雅娟..杂交三倍体泥鳅染色体C带及rDNA序列的FISH定位[J].水产学报,2018,42(2):178-185,8.基金项目
国家自然科学基金(31272650) （31272650）
辽宁省科学技术基金(201102019) （201102019）
National Natural Science Foundation of China (31272650) （31272650）
Natural Science Foundation of Liaoning Province (201102019) （201102019）
