水产学报2018，Vol.42Issue(2)：186-195,10.DOI:10.11964/jfc.20170110684
剑尾鱼趋化因子CCL4和CCL19基因的克隆及 嗜水气单胞菌感染对其组织表达的影响
Identification and expression analysis of two CC chemokines in swordtail fish (Xiphophorus helleri) responses to Aeromonas hydrophila challenges
摘要
Abstract
Chemokines are a large group of small proteins involved in migration, activation, and differentiation of leukocytes and are key regulators of the immune response and inflammatory response. In this study, we cloned and sequenced the cDNA of two CC chemokines (CCL4 and CCL19) from swordtail fish (X. helleri). The ORF-length ofCCL4 is 294 nucleotides (nt) encoding a protein of 97 amino acids (aa), and the ORF-length of CCL19 is 333 nucleotides (nt) encoding a protein of 110 amino acids (aa). Quantitative real-time RT-PCR (qRT-PCR) analysis revealed both two CC chemokines were constitutively expressed in all tissues examined. The CCL4 was highly ex-pressed in the spleen, lowly in the muscle and intestine; the CCL19 had the highest expression in the spleen and the relatively lower level in the heart. After challenge with bacteria, the CCL4 and CCL19 shared the same expression trend. In the spleen and liver, the mRNA level increased remarkably at 12 h, which suggested that the two CC chemokines may be involved in theX. helleri defense againstA. hydrophlia infection. In summary, this study demonstrates the basic profiles of two CC chemokines inX. helleri and chemokines take an important part in in-flammatory and immune responses to bacteria. Together, our results also indicate that theX. helleri could be util-ized as a useful fish model for the research of fish diseases.关键词
剑尾鱼/CCL4/CCL19/嗜水气单胞菌/克隆/表达分析Key words
Xiphophorus helleri/CCL4/CCL19/gene cloning/Aeromonas hydrophila/expression analysis分类
生物科学引用本文复制引用
朱成科,王建,陈灵涵,周朝伟,雷骆,邓星星,刘春,郑宗林..剑尾鱼趋化因子CCL4和CCL19基因的克隆及 嗜水气单胞菌感染对其组织表达的影响[J].水产学报,2018,42(2):186-195,10.基金项目
重庆市生态渔业技术体系建设专项(40800115, 40800216) （40800115, 40800216）
西南大学荣昌校区青年基金(20700913) （20700913）
The Ecological Fishery Industry Technology System of Chongqing (40800115, 40800216) （40800115, 40800216）
The Youth Foundation of Southwest University Rongchang Campus (20700913) （20700913）
