水产学报2018，Vol.42Issue(2)：196-203,8.DOI:10.11964/jfc.20161210645
三疣梭子蟹线粒体基因组单核苷酸多态性
Analysis of single nucleotide polymorphism (SNP) in mitochondrial genome of Portunus trituberculatus
摘要
Abstract
To obtain SNP mutations of mitochondrial genome in Portunus trituberculatus, a total of 179 primer pairs were designed, nearly covering the whole mitochondrial genome in this study. Of the 179 primer pairs, 89 got the expected specific amplification fragments by regular PCR. Then, 22 of 89 amplification fragments were found to contain the SNPs, by testing of HRM technology (High resolution melting curve). Though sequencing, 24 SNPs were obtained in the 22 amplification fragments. The results by statistical analyzing demonstrated that SNP fre-quency in mitochondrial genome ofP. trituberculatus was 0.15/100 bp, in which the conversion mutation rate was 79.1％, 16.6％ for transvesion mutation rate, 79.1％ for mutation rate of C to T (or G to A), 8.3％ for the mutation rate of G to T (or C to A), and 4.16 ％ for the mutation rate of G to C and A to T, respectively. Thus, the mutation of C to T (containing the type of G to A) is the most common mutation type. Additionally, the distribution of 24 SNP mutation loci was not even in the whole mitochondrial genome. The number of SNPs was the greatest in COX1 gene region, and the second is in D-LOOP and ND1 genes. However, no SNPs were found in some regions, such ast RNA, 12SrRNA. Our findings will provide an analysis tool that may help further researches in genetic di-versity of germplasm resources and enhancement in P. trituberculatus.关键词
三疣梭子蟹/线粒体基因组/SNP/高分辨率熔解曲线Key words
Portunus trituberculatus/mitochondrial genome/SNP/HRM分类
生物科学引用本文复制引用
赵莲,李志辉,张培,薛蓓,赖晓芳,高焕,阎斌伦..三疣梭子蟹线粒体基因组单核苷酸多态性[J].水产学报,2018,42(2):196-203,8.基金项目
国家自然科学基金(31472282) （31472282）
江苏省高校自然科学研究重大项目(13KJA240001) （13KJA240001）
江苏省"六大人才高峰"创新人才团队专项(2016-HYGC-CXTD-004) （2016-HYGC-CXTD-004）
National Science Foundation of China(31472282) （31472282）
Key University Science Research Project of Jiangsu Province (13KJA240001) （13KJA240001）
Projects of "Six Talent Summit" Innovative Talent Team of Jiangsu (2016-HY-GC-CXTD-004) （2016-HY-GC-CXTD-004）
