水产学报2018，Vol.42Issue(2)：204-215,12.DOI:10.11964/jfc.20170210712
拟穴青蟹Sp-Sox14基因在胚胎发育和性腺发育过程中的表达
Expression analysis of Sp-Sox14 gene during embryonic and gonadal development in Scylla paramamosain
摘要
Abstract
In this study, a full-length 2558 bpSp-Sox14 cDNA sequence was obtained from Scylla paramamosain gonadal transcriptome database. The encoded protein consists of 427 amino acids and includes an HMG box do-main. Phylogenetic tree analysis showed that Sp-Sox14 was closely clustered with Sox14 of other arthropods. Quantitative real-time PCR displayed thatSp-Sox14 had different expression levels in different tissues from ma-ture male and female crabs with the highest expression in ovary and the heart of male crabs. During embryogenes-is,Sp-Sox14 expression was the highest in eye-pigment formation stage. During the different stages of ovarian de-velopment,Sp-Sox14 was expressed at the highest level in previtellogenesis stage (O2) and which was signific-antly higher than other stages. During the testis development, the expression level of Sp-Sox14 was higher in ma-ture sperm stage (T3) than in spermatocyte stage (T1) and spermatid stage (T2). It was speculated that Sp-Sox14 was involved in the early development of ovary and the process of sperm maturation. The whole mountin situ hy-bridization (WISH) results indicated that the positive signals of Sp-Sox14 were mainly distributed in the head and near the maxillipede at eye-pigment formation stage, and near the compound eyes at prehatching stage. A small number of Sp-Sox14 positive signals were still present in the head and near the appendages at hatching stage. The results of WISH implied that Sp-Sox14 was involved in the formation of the nervous organ as well as related to the occurrence of somite appendages.关键词
拟穴青蟹/Sp-Sox14/胚胎发育/性腺发育/基因表达Key words
Scylla paramamosain/Sp-Sox14/embryogenesis/gonadal development/gene expression分类
生物科学引用本文复制引用
梁可蓥,张子平,廖佳倩,邹志华,王艺磊..拟穴青蟹Sp-Sox14基因在胚胎发育和性腺发育过程中的表达[J].水产学报,2018,42(2):204-215,12.基金项目
国家自然科学基金(31472266, 41676161, 31672681) （31472266, 41676161, 31672681）
National Science Foundation of China (31472266, 41676161, 31672681) （31472266, 41676161, 31672681）
