水产学报2018，Vol.42Issue(2)：236-245,10.DOI:10.11964/jfc.20170110677
团头鲂耐低氧F3的建立及其在低氧环境下的生长差异
Establishment of hypoxia-tolerant F3 generation of Megalobrama amblycephala and their growth performance under long-term hypoxia
摘要
Abstract
On the basis of wild good broodstocks introduced from Poyang Lake (F0 generation), the F1 generation was obtained through population breeding from 2009 to 2011, and the passage from F1 to F2 was realized in 2011 through population breeding. In order to construct hypoxia tolerance strains of M.amblycephala, the Poyang Lake F2 and "Pujiang No.1" F9 broodstocks endured twice hypoxia stress in the summer and autumn in 2012, and 536 survival individuals were constructed as hypoxia-tolerant F2 generation. In 2013, the F2 parents (female 50, male 48) with great body weight and good body-shape were used to establish 24 F3 family groups (22 groups of 2♀×2♂and 2 groups of 3♀×2♂), a total of 100 F3 families. The 100 F3 families were cultured under hypoxia for one year from the 20th day post-hatching. By measuring the corresponding traits between growth and hypoxia tolerance, five fastest growing family groups (A2, A3, A18, A19 and A20) were selected; microsatellite analysis identified these rapidly growing individuals belonging to 20 families. Additionally, six faster growing groups (A4, A6, A17, A25, A27 and A28) belonging to 28 family were identified. Our results show that the growth performance of M. amblycephala was positively correlated with hypoxia-tolerance. Under long-time hypoxia stress culture at the first-year stage of F3 families, the body weight of the families with strong hypoxia tolerance was significantly higher than that of the families with low hypoxia tolerance. The selected F3 families with hypoxia resistant characteristics still maintained rapid growth rates at 2-year-old stage. The purpose of this study is to establish the hypoxia-toler-ant F3 generation for subsequent selection of new good breed of M. amblycephala.关键词
团头鲂/家系群体/低氧胁迫/生长性状/耐低氧Key words
Megalobrama amblycephala/family selection/hypoxia stress/growth traits/hypoxia tolerance分类
农业科技引用本文复制引用
李福贵,郑国栋,吴成宾,陈杰,蒋霞云,邹曙明..团头鲂耐低氧F3的建立及其在低氧环境下的生长差异[J].水产学报,2018,42(2):236-245,10.基金项目
"十二五"国家科技支撑计划(2012BAD26B00) （2012BAD26B00）
国家自然科学基金(31572220) （31572220）
上海高校知识服务平台(ZF1206) （ZF1206）
Key Technologies Research and Development Program of China (2012BAD26B00) （2012BAD26B00）
National Science Foundation of China (31572220) （31572220）
Shanghai University Knowledge Service Platform (ZF1206) （ZF1206）
