四川中医2017，Vol.35Issue(12)：34-38,5.
滋水清肝饮对内分泌治疗后的乳腺癌移植小鼠脑内单胺类神经递质的作用
Effect of Zishui Qinggan Decoction on Monoamine Neurotransmitter in Brain of Transplanted Mice with Endocrine Therapy
摘要
Abstract
Objective:To observe the effect of Zishui Qingan Decoction on monoamine neumtransmitters in the brain after endocrine therapy for breast cancer transplanted mice,and to explore its mechanism to improve menopausal syndrome.Methods:5 × 1054T1 cells were inoculated in the second and lower breast fat cubes of the right abdomen of 40BALB/c female mice,and the model of breast cancer transplant was established.The postmenopausal breast cancer transplant was established by ovariectomy.The mice were randomly divided into 4groups:experiment group 1 (low dose group):Zishui Qingan Decoction 11.28g · kg-1+letrozole 0.379mg · kg-1;experimental group 2 (high dose group):Zishui Qingan Decoction 45.lg,kg-1 +letrozole 0.379mg ·kg-1;blank controlled group:equal volume of distilled water;positive controlled group:letrozole 0.379mg · kg-1,after estrous administration for 4weeks,the levels of estradiol (E2),follicle stimulating hormone (FSH) and luteinizing hormone (LH) were measured by enzyme-linked immunosorbent assay (Elisa);The contents of 5-HT1AR and 5-HT2AR protein were detected by Westem blotting;The expression of 5-HT1AR and 5-HT2AR protein were detected by Western blotting.Results:Elisa method showed that the dose of E2,FSH and LH in mice was significantly lower than that in the positive controlled group (P＜0.05),and the levels of 5-HT and NE (P＜0.05).Compared with the model group and the positive controlled group,the expression of 5-HT1AR increased and the expression of 5-HT2AR was decreased (P＜0.05),and the expression of 5-HT1AR in the brain was higher than that in the blank controlled group and positive controlled group,and the effect of high dose group was more obvious (P＜0.05).Conclusion:Zishui Qinggan Decoction may relieve endocrine therapy-related menopausal syndrome by modulating monoamine neurotransmitters.关键词
滋水清肝饮/乳腺癌/移植小鼠/单胺类神经递质/绝经期综合征Key words
Zishui Qingan Decoction/Breast cancer/Transplanted mice/Monoamine neurotransmitters/Menopausal syndrome分类
医药卫生引用本文复制引用
刘林涛,胡灿红,霍介格,李灵常..滋水清肝饮对内分泌治疗后的乳腺癌移植小鼠脑内单胺类神经递质的作用[J].四川中医,2017,35(12):34-38,5.基金项目
江苏省中医药管理局立项课题(编号:LZ13077). （编号:LZ13077）
