山东医药2017，Vol.57Issue(37)：9-11,3.DOI:10.3969/j.issn.1002-266X.2017.37.003
大黄含药血清对乳鼠原代肝细胞能量代谢的影响
Effect of serum containing Dahuang (rhubarb) on energy metabolism of rat primary hepatocytes
摘要
Abstract
Objective To observe the effect of serum containing Dahuang (rhubarb) on energy metabolism of rat primary hepatocytes.Methods Medicated serum was prepared on SD rats.Hepatocytes were isolated from SD neonatal rats by collagenase digestion and then were randomly divided into the blank serum group and medicated serum group.Cells in the two groups were cultured with 20％ blank serum and 20％ serum containing Dahuang (rhubarb) for 24 hours,respectively.The activities of Na+-K +-ATPase,and Ca2+-Mg2+-ATPase were detected with spectrophotometer.The content of ATP,ADP,and AMP was tested with high performance liquid chromatography (HPLC) as well as energy charge.The mitochondrial membrane potential was analyzed with rhodamine 123 by laser confocal microscope.Results Compared with the blank serum group,the activities of Na +-K+-ATPase and Ca2+-Mg2+-ATPase decreased (all P ＜ 0.05) and the content of ATP decreased in the medicated serum group (P ＜ 0.05).There was no significant difference in the level of energy charge between the two groups (P ＞ 0.05).Compared with the blank serum group,the mitochondrial membrane potential decreased in the medicated serum group (P ＜ 0.05).Conclusion The serum containing Dahuang (rhubarb) may inhibit the energy metabolism and reduce the energy reserve of rat primary hepatocytes by regulating mitochondrial function.关键词
肝细胞/能量代谢/大黄含药血清/中药/大鼠Key words
hepatocytes/energy metabolism/serum containing Dahuang (rhubarb)/traditional Chinese medicine/rats分类
医药卫生引用本文复制引用
阚东方,季旭明,陈倩,韩晓春,武继彪..大黄含药血清对乳鼠原代肝细胞能量代谢的影响[J].山东医药,2017,57(37):9-11,3.基金项目
国家重点基础研究发展计划(2007CB512601,2013CB531803) （2007CB512601,2013CB531803）
国家自然科学基金面上项目(81673852) （81673852）
山东省重点产业关键技术项目(2016CYJS08A01-4,2016CYJS08A01-3). （2016CYJS08A01-4,2016CYJS08A01-3）
