山东医药2017，Vol.57Issue(37)：12-14,3.DOI:10.3969/j.issn.1002-266X.2017.37.004
细胞周期调控基因及Bcl-2、c-Jun基因在人肝细胞脂肪变性过程中的表达变化
Expression changes of cell cycle regulatory genes, Bcl-2, and c-Jun in the process of human hepatic steatosis
摘要
Abstract
Objective To investigate the expression changes of cell cycle regulatory genes [Cyclin-dependent kinase2 (CDK2),Cyclin-dependent kinase4 (CDK4),and CyclinD1],B-cell lymphoma/leukemia-2 (Bcl-2),and c-Jun in the process of human hepatic steatosis.Methods L02 liver cell line was incubated with mixture of oleic acid and palmitic acid by the ratio of 2 ∶ 1 (OP21) for 0,5,10,and 20 h to build the models of human hepatocyte steatosis.Oil Red O staining was performed to evaluate the fat content of the L02 liver cells.The mRNA expression levels of CDK2,CDK4,CyclinD1,Bcl-2,and c-Jun in the L02 cells at different time points were detected by real-time PCR.Results More and more orange droplets were accumulated in the L02 cells with the time goes on,and the liver cells were dyed orange at 20 h.The mRNA expression level of CDK2 increased gradually,reached the peak at 5 h (P ＜ 0.05),and then decreased gradually;the mRNA expression level of CDK4 increased gradually,reached the peak at 10 h (P ＞ 0.05),then decreased gradually,and reached the lowest point at 20 h (P ＞ 0.05);no statistically significant difference was found in the expression levels of Cyclin D1 at different time points during the process (all P ＞ 0.05);the mRNA expression level of Bcl-2 increased over time,reached the peak at 10 h (P ＜0.05),and then decreased;the mRNA expression level of c-Jun was higher at 5,10,and 20 h than that at 0 h (all P ＜ 0.05),but there were no regular changes.Conclusion During the process of hepatic steatosis,the relative mRNA levels of the cancer-related genes (CDK2,CDK4,Bcl-2,and c-Jun) first increase and then decrease,while the expression of Cyclin D1 does not change significantly.关键词
肝细胞脂肪变性/细胞周期/细胞周期蛋白/细胞周期蛋白依赖性激酶/细胞周期蛋白依赖性激酶抑制因子/B细胞白血病-淋巴瘤基因2/c-Jun基因Key words
hepatic steatosis/cell cycle/Cyclin/Cyclin-dependent kinase/Cyclin-dependent kinase inhibitor/B-cell lymphoma/leukemia-2/c-Jun gene分类
医药卫生引用本文复制引用
包成,毕玉晶,杨瑞馥,智发朝..细胞周期调控基因及Bcl-2、c-Jun基因在人肝细胞脂肪变性过程中的表达变化[J].山东医药,2017,57(37):12-14,3.基金项目
国家高技术研究发展计划(863计划)(2015AA020702). （863计划）
